Description

Hygromycin B is a potent aminoglycoside antibiotic derived from *Streptomyces hygroscopicus*. Its primary value lies in its selective toxicity against prokaryotic cells, making it an essential agent for research and industrial applications requiring the elimination of bacterial contaminants. This product is particularly critical for the biotechnology and pharmaceutical industries, where it is used as a selective agent in cell culture and molecular biology workflows. Global suppliers and research institutions rely on consistent, high-purity Hygromycin B to ensure the integrity of their scientific and production processes.

Application

• Selective Agent in Cell Culture: Used to select and maintain eukaryotic cells (e.g., mammalian, plant, yeast) that have been transfected with a vector carrying the hygromycin resistance gene.
• Molecular Biology Research: A critical component in genetic engineering experiments for stable transfection and the generation of stable cell lines.
• Antibiotic Research: Serves as a reference standard and active ingredient in studies investigating aminoglycoside antibiotics, their mechanisms of action, and resistance.
• Veterinary and Agricultural Research: Historically used as an anthelmintic and for growth promotion in animal husbandry; now primarily a tool in related research fields.
• Microbiological Media Supplement: Incorporated into agar or broth media to create selective conditions for the growth of specific, resistant microorganisms in industrial fermentation or quality control.
• Biopharmaceutical Production: Employed in the development and manufacturing processes of therapeutic proteins and vaccines where selective pressure is required during cell line development.

Basic Information

Product Name	Hygromycin B
CAS No.	8052-93-5
Molecular Formula	C20H37N3O13
Molecular Weight	527.52 g/mol
Synonyms	Hygromycin; Hygromix; Hygromisin; O-De(2-amino-2-deoxy-α-D-glucopyranosyl)hygromycin A; Antibiotic XK-49; Streptomyces hygroscopicus antibiotic; NSC 529634; Antibiotic 172
EINECS	Contact for details

Quality Control

Our Hygromycin B is produced and tested under strict quality management systems to ensure it meets the high standards required for research and industrial use. Each batch is subjected to comprehensive analytical testing, including identity confirmation, potency (assay), and purity profiling. A Certificate of Analysis (COA) detailing specific results for parameters such as purity (by HPLC), water content, and residual solvents is provided with every shipment. We adhere to relevant industry guidelines to guarantee product consistency and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0% (anhydrous basis)
Water (Karl Fischer)	≤ 5.0 %
pH (1% solution)	5.0 - 7.5
Specific Rotation	+110° to +120° (c=1 in H2O)
Biological Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.