Description

Kanamycin Sulfate is a broad-spectrum aminoglycoside antibiotic derived from *Streptomyces kanamyceticus*. It is a critical tool for inhibiting bacterial protein synthesis, making it highly effective against a wide range of Gram-negative and some Gram-positive bacteria. This compound is essential for research and development in microbiology, molecular biology, and pharmaceutical manufacturing. Kanamycin Sulfate CAS NO 133-92-6 is a fundamental reagent for applications such as cell culture selection and the study of bacterial resistance mechanisms.

Application

• Cell Culture & Microbiology Research: Widely used as a selective agent in cell culture media, particularly for the selection of eukaryotic cells transfected with plasmids containing the kanamycin resistance gene (e.g., pET vectors).
• Pharmaceutical Intermediates: Serves as a key active pharmaceutical ingredient (API) and intermediate in the production of injectable antibiotic formulations.
• Molecular Biology: Employed in bacterial transformation procedures to select for recombinant bacteria, making it indispensable for cloning, protein expression, and genetic engineering workflows.
• Veterinary Medicine: Used in the formulation of treatments for bacterial infections in livestock and companion animals.
• Agricultural Research: Applied in plant tissue culture and transgenic plant research as a selective agent.
• Biochemical Research: Utilized in studies of bacterial ribosome function, antibiotic mechanisms of action, and resistance pathways.

Basic Information

Product Name	Kanamycin Sulfate
CAS No.	133-92-6
Molecular Formula	C18H36N4O11 • H2SO4
Molecular Weight	582.58 g/mol (for the disulfate form)
Synonyms	Kanamycin Monosulfate; Kanamycin A Sulfate; Kanasig; Kanamycin Sulfate Salt; Kantrex; 6-O-(3-Amino-3-deoxy-α-D-glucopyranosyl)-4-O-(6-amino-6-deoxy-α-D-glucopyranosyl)-2-deoxy-D-streptamine Sulfate; O-3-Amino-3-deoxy-α-D-glucopyranosyl-(1→6)-O-[6-amino-6-deoxy-α-D-glucopyranosyl-(1→4)]-2-deoxy-D-streptamine Sulfate
EINECS	205-138-7

Quality Control

Our Kanamycin Sulfate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for research and pharmaceutical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming specifications for identity, potency, purity, and microbiological quality. We can supply material compliant with various pharmacopeial standards (e.g., USP, EP) upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment to maintain stability and potency. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White or almost white powder
Identification (IR)	Conforms
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 750 µg/mg (as Kanamycin base)
pH (10% solution)	6.0 - 8.0
Loss on Drying	≤ 5.0 %
Sulfate Content	Conforms
Residue on Ignition	≤ 1.0 %
Heavy Metals	≤ 20 ppm
Bacterial Endotoxins	< 0.5 EU/mg (for injectable grade)
Sterility	Sterile (for sterile-filtered grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.