Description

Bis(Nhs)Peg5 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring two N-hydroxysuccinimide (NHS) ester termini. This compound is specifically engineered for the efficient and stable conjugation of amine-containing molecules, such as proteins, peptides, and antibodies, in aqueous or buffered solutions. It is a critical reagent for researchers and manufacturers in the fields of bioconjugation, proteomics, and diagnostic assay development, where controlled and reproducible crosslinking is essential.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a cleavable or non-cleavable spacer to connect cytotoxic payloads to monoclonal antibodies.
• Protein-Polymer Conjugation: Used to PEGylate therapeutic proteins and peptides to enhance solubility, stability, and circulation half-life.
• Biomolecule Immobilization: Facilitates the covalent attachment of proteins, enzymes, or oligonucleotides to solid surfaces (e.g., beads, microplates, biosensors) for assay and diagnostic kit development.
• Crosslinking Agent: Enables the formation of stable amide bonds between different amine-containing biomolecules for studying protein-protein interactions or creating novel biomaterials.
• Fluorescent Probe Labeling: Conjugates amine-modified fluorescent dyes or tags to antibodies or other targeting molecules for imaging and flow cytometry applications.
• Nanoparticle Functionalization: Used to attach targeting ligands or therapeutic agents to the surface of nanoparticles for drug delivery systems.

Basic Information

Product Name	Bis(Nhs)Peg5
CAS No.	756526-03-1
Molecular Formula	C26H40N2O14
Molecular Weight	604.61 g/mol
Synonyms	Bis-NHS-PEG5; Bis(succinimidyl) PEG5; Bis(NHS) PEG5 ester; NHS-PEG5-NHS; 1,11-Bis[(2,5-dioxopyrrolidin-1-yl)oxy]-3,6,9-trioxaundecane; PEG5 bis(N-hydroxysuccinimide ester); Bis-N-hydroxysuccinimide PEG5; Succinimidyl PEG5 succinimidyl ester
EINECS	Contact for details

Quality Control

Our Bis(Nhs)Peg5 is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive life science applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group analysis. We adhere to ISO 9001 quality management principles, and our production can be aligned with cGMP standards for pharmaceutical development upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester groups.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.