Description

γ-tert-Butyl Methotrexate is a chemically modified derivative of the established pharmaceutical agent methotrexate, designed to offer enhanced properties for advanced research and development. This compound is of significant interest for its potential to serve as a key intermediate or a prodrug candidate in the synthesis of novel therapeutic agents. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and fine chemical manufacturers focused on oncology and autoimmune disease research. Its specific structure allows for targeted exploration of improved drug delivery and metabolic stability.

Application

• Pharmaceutical Intermediate: A crucial building block in the research and synthesis of novel methotrexate analogs and targeted cancer therapies.
• Prodrug Development: Investigated for its potential as a prodrug, designed to improve bioavailability or achieve site-specific activation of methotrexate.
• Biochemical Research: Used in studies exploring structure-activity relationships (SAR) of antifolate drugs to understand and enhance their mechanism of action.
• Reference Standard: Serves as a high-purity analytical standard for quality control and method development in pharmaceutical analysis (HPLC, LC-MS).
• Academic Research: Employed in university and institutional labs for fundamental research in medicinal chemistry and drug design.
• API Synthesis: A potential precursor in the controlled synthesis of Active Pharmaceutical Ingredients (APIs) for experimental formulations.

Basic Information

Product Name	γ-tert-Butyl Methotrexate
CAS No.	79640-76-9
Molecular Formula	C25H31N7O5
Molecular Weight	509.56 g/mol
Synonyms	N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-γ-(tert-butyl)-L-glutamic Acid; tert-Butyl Methotrexate; MTX tert-Butyl Ester; γ-(tert-Butyl) Methotrexate; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, γ-(1,1-dimethylethyl) ester; Methotrexate γ-tert-butyl ester
EINECS	Contact for details

Quality Control

Our γ-tert-Butyl Methotrexate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. Standard specifications typically include assessment by HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, related substances, and residual solvents is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.