Description

n-(Prednisolone-21-Succinylaminohexyl)-N'-Fluoresceinyl-Thiourea is a sophisticated fluorescent-labeled steroid derivative designed for advanced research and diagnostic applications. This compound matters because it combines the potent glucocorticoid activity of prednisolone with the high-visibility detection capabilities of fluorescein, enabling precise tracking and analysis in biological systems. It is primarily needed by pharmaceutical researchers, biochemists, and diagnostic developers working in drug discovery, receptor binding studies, and high-sensitivity immunoassays.

Application

• Biochemical Research & Drug Discovery: Used as a high-affinity fluorescent tracer for glucocorticoid receptor (GR) binding assays and cellular uptake studies.
• Immunoassay Development: Serves as a critical hapten or labeled antigen in the development of fluorescence-based immunoassays (FIA) and enzyme-linked immunosorbent assays (ELISA) for steroid detection.
• Cellular Imaging & Microscopy: Enables visualization and localization of steroid pathways and receptor interactions in live or fixed cells using fluorescence microscopy.
• Pharmaceutical Analysis: Employed as an analytical reference standard in method development and quality control for prednisolone and related corticosteroid formulations.
• Diagnostic Probe Synthesis: Acts as a key intermediate for synthesizing more complex diagnostic probes and targeted imaging agents.

Basic Information

Item	Detail
Product Name	n-(Prednisolone-21-Succinylaminohexyl)-N'-Fluoresceinyl-Thiourea
CAS No.	78232-37-8
Molecular Formula	C52H60N4O11S
Molecular Weight	949.13 g/mol
Synonyms	Prednisolone-21-hemisuccinate-6-aminohexylamide-fluorescein thiourea; Fluorescein-labeled prednisolone; Prednisolone-fluorescein conjugate; 21-Dehydroxyprednisolone 21-hemisuccinamidohexylamide fluorescein thiourea; Glucocorticoid-fluorescein probe; Fluorescent glucocorticoid derivative; FITC-Prednisolone conjugate (common name); Research compound 78232-37-8
EINECS	Contact for details

Quality Control

Every batch of n-(Prednisolone-21-Succinylaminohexyl)-N'-Fluoresceinyl-Thiourea is produced and controlled under strict quality management systems. We ensure high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and conformity to customer specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, freezer environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.