Description

n-Formyl-Nle-Leu-Phe Methyl Ester is a synthetic tripeptide derivative, specifically a formylated and methylated analog of the chemotactic peptide fMLF. This compound is a crucial biochemical tool for studying formyl peptide receptor (FPR) signaling pathways and neutrophil chemotaxis. It is primarily utilized by researchers in pharmaceutical R&D, biochemistry, and immunology for investigating inflammatory responses and developing novel therapeutic agents.

Application

• Biochemical Research: A standard ligand for formyl peptide receptor (FPR) binding and activation studies.
• Immunology Studies: Used to investigate neutrophil chemotaxis, degranulation, and superoxide production in inflammatory models.
• Pharmaceutical Screening: Serves as a reference compound or agonist in high-throughput screening assays for anti-inflammatory drug discovery.
• Signal Transduction Research: A key tool for elucidating G-protein coupled receptor (GPCR) signaling mechanisms downstream of FPR activation.
• Peptide Chemistry: Used as an intermediate or reference standard in the synthesis and analysis of more complex peptide analogs.
• Academic & Institutional Research: Fundamental reagent for university and government lab studies in cell biology and innate immunity.

Basic Information

Product Name	n-Formyl-Nle-Leu-Phe Methyl Ester
CAS No.	77542-99-5
Molecular Formula	C26H39N3O6
Molecular Weight	489.61 g/mol
Synonyms	Formyl-Norleucyl-Leucyl-Phenylalanine Methyl Ester; f-Nle-Leu-Phe-OMe; N-Formyl-L-norleucyl-L-leucyl-L-phenylalanine Methyl Ester; For-Nle-Leu-Phe-OMe; Chemotactic Peptide fNLPNTL Analog; Formyl-Met-Leu-Phe (fMLF) Analog; Formyl Peptide Receptor Agonist; N-Formyl-Met-Leu-Phe Methyl Ester Analog
EINECS	Contact for details

Quality Control

Our n-Formyl-Nle-Leu-Phe Methyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and chiral analysis to ensure stereochemical integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee consistent quality and batch-to-batch reproducibility for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.