Description

H-Ala-Amc CAS NO 77471-41-1 is a specialized protected amino acid derivative, specifically an alanine amide with a C-terminal 7-amino-4-methylcoumarin (AMC) fluorophore. This compound is a critical building block and substrate in biochemical research and pharmaceutical development. It is primarily utilized by researchers and manufacturers in the fields of protease enzymology, high-throughput screening, and peptide synthesis. The AMC moiety makes it an essential tool for developing sensitive fluorogenic assays to study enzyme kinetics and inhibitor activity.

Application

• Fluorogenic Protease Substrate: Key reagent for continuous assays targeting various proteases, including aminopeptidases and specific serine or cysteine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel enzyme inhibitors for therapeutic targets.
• Biochemical Research: Fundamental tool for studying enzyme mechanisms, kinetics, and specificity in academic and industrial laboratories.
• Diagnostic Kit Development: Employed in the formulation of sensitive diagnostic assays for detecting enzymatic activity related to specific disease states.
• Peptide Synthesis Intermediate: Serves as a protected building block in the synthesis of more complex peptide-fluorophore conjugates.

Basic Information

Product Name	H-Ala-Amc
CAS No.	77471-41-1
Molecular Formula	C₁₃H₁₄N₂O₃
Molecular Weight	246.26 g/mol
Synonyms	L-Alanine 7-amido-4-methylcoumarin; Ala-AMC; Alanine AMC; H-L-Ala-AMC; L-Ala-7-amido-4-methylcoumarin; 7-(L-Alanylamido)-4-methylcoumarin; N-(7-Amino-4-methylcoumarinyl)-L-alaninamide; Fluorogenic substrate Ala-AMC
EINECS	Contact for details

Quality Control

Our H-Ala-Amc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, identity confirmation by spectroscopic methods, and verification of fluorogenic activity. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional (Lot-specific data on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.