Description

cis-4-Hydroxy-D-Proline Hydrochloride is a high-purity, non-proteinogenic amino acid derivative of significant interest in advanced pharmaceutical research and development. This chiral building block is valued for its role in modulating the conformational stability and biological activity of peptide-based therapeutics. It is primarily utilized by research institutions and pharmaceutical companies engaged in the synthesis of novel peptide drugs, enzyme inhibitors, and other bioactive compounds where precise stereochemistry is critical.

Application

• Pharmaceutical Intermediate: A key chiral synthon in the synthesis of peptide-based active pharmaceutical ingredients (APIs) and prodrugs.
• Protease Inhibitor Development: Used in research for designing inhibitors targeting collagenases and other hydroxyproline-specific enzymes.
• Conformational Studies: Employed to introduce structural constraints and stabilize specific secondary structures (e.g., polyproline type II helices) in synthetic peptides.
• Biochemical Research: Serves as a standard or reagent in studies of collagen biosynthesis, post-translational modification, and related metabolic pathways.
• Ligand Synthesis: Used in the preparation of chiral ligands for asymmetric catalysis and metal complexation.
• Cell Culture Media Component: May be utilized as a specialized component in certain advanced cell culture and tissue engineering applications.

Basic Information

Product Name	cis-4-Hydroxy-D-Proline Hydrochloride
CAS No.	77449-94-6
Molecular Formula	C5H9NO3·HCl
Molecular Weight	167.59 g/mol (Free base: 131.13)
Synonyms	(2R,4R)-4-Hydroxypyrrolidine-2-carboxylic acid hydrochloride; D-4-Hydroxyproline hydrochloride; (R,R)-4-Hydroxyproline hydrochloride; trans-4-Hydroxy-L-proline enantiomer hydrochloride; 4(R)-Hydroxy-D-proline hydrochloride; cis-4-Hydroxy-D-proline HCl; HYP(D)-HCl
EINECS	Contact for details

Quality Control

Our cis-4-Hydroxy-D-Proline Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including chiral purity verification by HPLC, to ensure it meets the stringent requirements for pharmaceutical research. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0% de
Loss on Drying	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.