Description

Black Mamba Protein A is a high-purity, affinity chromatography ligand derived from *Staphylococcus aureus*. This product is essential for the efficient and specific purification of antibodies and antibody fragments in biopharmaceutical manufacturing. It is a critical reagent for research, diagnostic kit development, and large-scale therapeutic protein production. Industries requiring high-purity immunoglobulin isolation, such as biopharma, contract research organizations (CROs), and in-vitro diagnostics (IVD), rely on its consistent performance.

Application

• Monoclonal Antibody (mAb) Purification: Primary application for capturing IgG from cell culture supernatants in downstream processing.
• Research Scale Antibody Isolation: Used in laboratory settings for purifying antibodies from serum, ascites fluid, or hybridoma culture.
• Immunoassay and Diagnostic Kit Manufacturing: Serves as a key component for immobilizing antibodies on solid supports for ELISA, lateral flow assays, and biosensors.
• Affinity Column Packing: Immobilized onto agarose, sepharose, or other matrices to create prepacked or custom chromatography columns.
• Fc-Fusion Protein Purification: Captures proteins engineered with the Fc region of immunoglobulins.
• Immunoprecipitation (IP) and Co-Immunoprecipitation (Co-IP): A critical tool for protein complex isolation and analysis in proteomics and molecular biology research.

Basic Information

Item	Detail
Product Name	Black Mamba Protein A
CAS No.	76723-44-9
Molecular Formula	Contact for details
Molecular Weight	Approx. 42 kDa (Protein A monomer)
Synonyms	Protein A (Staphylococcal); Staphylococcal Protein A; SpA; IgG-binding Protein A; Affinity Chromatography Ligand A; Immunoglobulin G Binding Protein; Recombinant Protein A (if applicable); SPA
EINECS	Contact for details

Quality Control

Our Black Mamba Protein A is manufactured under strict quality systems. Each batch undergoes rigorous testing to ensure high purity, binding capacity, and low endotoxin levels, suitable for research and bioprocessing applications. A comprehensive Certificate of Analysis (COA) documenting identity, purity, biological activity, and specification compliance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Purity (SDS-PAGE)	≥ 95%
Assay (IgG Binding)	≥ 95% of theoretical capacity
Endotoxin (LAL)	< 10 EU/mg
Moisture Content (KF)	< 5.0 %
Solubility	Soluble in water and standard buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.