Description

Oxamate-Diaminohexyl-Sepharose is a specialized affinity chromatography medium designed for the purification of lactate dehydrogenase (LDH) and related enzymes. This product matters for researchers and manufacturers requiring high-purity enzyme isolation for diagnostic, pharmaceutical, and biochemical research applications. It is needed by biotechnology companies, academic research institutions, and diagnostic kit manufacturers engaged in enzymology, metabolic studies, and bioprocess development.

Application

• Affinity Purification of Lactate Dehydrogenase (LDH): Primary application for isolating LDH isoenzymes from complex biological mixtures such as cell lysates, serum, or fermentation broths.
• Enzyme Inhibition Studies: Used as an immobilized ligand platform for studying oxamate-based enzyme inhibitors and their kinetics.
• Diagnostic Reagent Manufacturing: Critical in the production of high-purity LDH enzymes for clinical diagnostic assays and test kits.
• Metabolic Pathway Research: Facilitates the study of glycolysis and anaerobic metabolism by enabling the specific capture of key enzymes.
• Bioprocess Development: Employed in downstream processing for the scalable purification of recombinant LDH expressed in microbial or mammalian systems.
• Proteomics and Biomarker Discovery: Useful for selectively binding and identifying LDH and its interacting protein partners in complex proteomic samples.

Basic Information

Product Name	Oxamate-Diaminohexyl-Sepharose
CAS No.	76082-51-4
Molecular Formula	N/A (Functionalized Chromatography Matrix)
Molecular Weight	N/A (Matrix-based product)
Synonyms	Oxamate-Sepharose 4B; N6-(2-Aminoethyl)-NAD-Agarose analog; LDH-Sepharose; Sepharose-Oxamate; Hexanediamine-Sepharose-Oxamate; Oxamic acid–Sepharose CL-4B; Affi-Gel Oxamate; Lactate Dehydrogenase Affinity Gel
EINECS	Contact for details

Quality Control

Our Oxamate-Diaminohexyl-Sepharose is produced under stringent quality protocols to ensure batch-to-batch consistency and performance. Each lot is tested for ligand density, binding capacity for LDH, matrix stability, and absence of microbial contamination. Certificates of Analysis (COA) detailing these parameters are available upon request. Our manufacturing aligns with ISO 9001 quality management principles to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store as a 20% ethanol slurry at 2-8°C. Do not freeze. Under these conditions, the product is stable for at least two years from the date of receipt.

Specification

Item	Specification
Matrix	Sepharose 4B
Ligand	Oxamate linked via diaminohexyl spacer
Form	Pre-swollen beads in suspension
Binding Capacity	> 5 mg LDH per mL settled gel
Ligand Density	5 - 15 μmol/mL settled gel
Particle Size	45 - 165 μm
Storage Buffer	20% Ethanol
pH Stability	3 - 10 (long term); 2 - 12 (short term)
Microbial Bioburden	< 10 CFU/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.