Description

n-Cbz-Leu-Leu-Glu β-Naphthylamide is a synthetic, chromogenic peptide substrate designed for enzymatic activity studies. This compound matters as a specialized tool for the sensitive detection and quantification of proteolytic enzyme activity, particularly in research settings. It is primarily needed by biochemical researchers, pharmaceutical developers, and diagnostic kit manufacturers working in enzymology and protease inhibitor screening.

Application

• Protease & Peptidase Research: Used as a specific chromogenic substrate for studying the kinetics and inhibition of various proteolytic enzymes.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for identifying and characterizing novel protease inhibitors as potential drug candidates.
• Diagnostic Assay Development: Serves as a key component in developing colorimetric or fluorometric assays for clinical diagnostics and biochemical testing.
• Enzyme Characterization: Assists in determining enzyme specificity, optimal pH, and kinetic parameters (Km, Vmax) for research purposes.
• Biochemical Reagent: Used as a premium-grade reagent in life science research laboratories for precise experimental work.
• Quality Control in Biotechnology: Applied in the QC testing of enzyme preparations and related biopharmaceutical products.

Basic Information

Product Name	n-Cbz-Leu-Leu-Glu β-Naphthylamide
CAS No.	75873-85-7
Molecular Formula	C₃₄H₄₄N₄O₈
Molecular Weight	636.74 g/mol
Synonyms	Carbobenzoxy-Leucyl-Leucyl-Glutamic Acid β-Naphthylamide; Z-Leu-Leu-Glu-βNA; CBZ-Leu-Leu-Glu-2-Naphthylamide; N-Carbobenzyloxy-L-leucyl-L-leucyl-L-glutamic acid 2-naphthylamide; Leu-Leu-Glu-βNA, Cbz-; Protease Substrate 75873-85-7; Chromogenic Peptide Substrate; L-Leucine, N-[(1S)-1-[[(2S)-1-[[(1S)-1-[(2-naphthalenylamino)-1-oxo-4-carboxybutyl]amino]-4-methyl-1-oxopentyl]amino]-4-methyl-1-oxopentyl]carbamic acid phenylmethyl ester
EINECS	Contact for details

Quality Control

Our n-Cbz-Leu-Leu-Glu β-Naphthylamide is manufactured under controlled conditions and undergoes rigorous quality testing to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and related substance profiles are available upon request. While not intended for human therapeutic use, production adheres to principles of good laboratory practice.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Assay (HPLC)	≥ 95.0%
Water Content	≤ 5.0% (KF)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.