Description

Ribosyl-Diphthamide CAS NO 75652-53-8 is a specialized nucleotide derivative and a key intermediate in the biosynthesis of diphthamide, a unique post-translationally modified histidine residue found in eukaryotic translation elongation factor 2 (eEF2). This compound is essential for researchers and manufacturers in the life sciences sector, particularly for studying protein synthesis, cellular toxicity mechanisms, and post-translational modification pathways. It is primarily utilized by biopharmaceutical R&D teams, academic research institutions, and diagnostic developers focused on enzymology, molecular biology, and targeted therapeutic discovery.

Application

• Biochemical Research: Critical substrate for in vitro studies of diphthamide biosynthesis enzymes, including diphthamide synthase components.
• Toxin Mechanism Studies: Used to investigate the action of ADP-ribosylating toxins like diphtheria toxin and Pseudomonas exotoxin A, which target eEF2.
• Enzyme Assay Development: Serves as a standard or reagent in high-throughput screening assays for identifying inhibitors of diphthamide formation or toxin activity.
• Post-Translational Modification Analysis: Employed in metabolic labeling experiments to track and analyze the diphthamide modification pathway in cells.
• Pharmaceutical R&D: A valuable intermediate for developing novel therapeutic agents that modulate protein synthesis or target the eEF2 pathway in cancers or other diseases.
• Diagnostic Reagent Manufacturing: Potential use in producing components for diagnostic kits that detect specific bacterial toxins or related cellular responses.

Basic Information

Product Name	Ribosyl-Diphthamide
CAS No.	75652-53-8
Molecular Formula	C20H32N6O12P2
Molecular Weight	618.46 g/mol
Synonyms	Diphthamide Ribosyl Phosphate; 2'-(5''-Adenosyl)diphthamide; ADP-Ribosyl-Diphthamide; Diphthine ADP-Ribose Ester; 5'-O-{N2-[2-(3-Carbamoyl-3-trimethylammoniopropyl)histidino]}-ADP-ribose; AHD riboside; (ADP-Ribosyl)diphthamide
EINECS	Contact for details

Quality Control

Our Ribosyl-Diphthamide is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, temperature-controlled environment. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.