Description

Testosterone-Dah-Fluorescein is a specialized fluorescent-labeled steroid derivative, designed for high-sensitivity detection and analysis. This compound matters for researchers and quality control professionals who require precise, trace-level quantification of testosterone or related analytes in complex matrices. It is primarily needed by the pharmaceutical, biotechnology, and clinical diagnostics industries for applications in assay development, bioimaging, and analytical method validation.

Application

• Fluorescent Immunoassays: Acts as a critical tracer or conjugate in fluorescence polarization immunoassays (FPIA) and other homogeneous assay formats for hormone detection.
• Analytical Reference Standard: Serves as a certified reference material (CRM) for the calibration and validation of HPLC, LC-MS/MS, and other chromatographic methods analyzing testosterone.
• Cellular & Molecular Imaging: Used as a probe in fluorescence microscopy to study steroid hormone receptor localization, trafficking, and activity in live or fixed cells.
• Diagnostic Kit Development: A key component in the development of in-vitro diagnostic (IVD) kits and point-of-care testing devices for endocrine function and sports doping control.
• Biochemical Research: Employed in competitive binding studies, receptor-ligand interaction analysis, and other fundamental research exploring androgen pathways.
• Pharmaceutical QC: Utilized in quality control laboratories for the impurity profiling and stability testing of testosterone-based pharmaceutical products.

Basic Information

Product Name	Testosterone-Dah-Fluorescein
CAS No.	75585-82-9
Molecular Formula	C44H48N2O7
Molecular Weight	716.87 g/mol
Synonyms	Testosterone-3-(O-carboxymethyl)oxime-fluorescein conjugate; Testosterone-FITC; Testosterone-fluorescein isothiocyanate derivative; 17β-Hydroxyandrost-4-en-3-one-fluorescein label; Fluorescein-labeled testosterone; Androgen-fluorescein probe; Testosterone O-(Carboxymethyl)oxime Fluorescein Amide
EINECS	Contact for details

Quality Control

Every batch of Testosterone-Dah-Fluorescein is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting identity, purity, and related substance profiles. Our quality commitment aligns with standards relevant for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This material is strictly light-sensitive and must be protected from all light exposure during handling and storage. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	≤ 5.0% total
Fluorescence Properties	Ex/Em: ~494 nm / ~518 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.