Description

D-Valyl-L-Phenylalanyl-n-(4-Nitrophenyl)-L-Lysinamide Dihydrochloride is a synthetic, modified tripeptide derivative of significant interest in advanced biochemical and pharmaceutical research. This compound is valued for its role as a specialized building block or a potential enzyme substrate/inhibitor in the development of novel therapeutic agents and diagnostic tools. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in peptide chemistry, protease studies, and drug discovery programs.

Application

• Pharmaceutical Research & Development: Serves as a key intermediate or a model substrate in the synthesis and study of novel peptide-based drugs, particularly those targeting specific enzymatic pathways.
• Protease/Enzyme Studies: Used as a specialized substrate to investigate the activity, specificity, and inhibition mechanisms of proteolytic enzymes like trypsin, plasmin, or other serine proteases.
• Biochemical Assay Development: Employed in the design and validation of high-throughput screening (HTS) assays and diagnostic kits for detecting enzyme activity or screening potential inhibitors.
• Peptide Chemistry & Conjugation: Acts as a building block for constructing more complex peptide structures or for conjugation with other molecules (e.g., fluorophores, carriers) in bioconjugation projects.
• Academic Research: Utilized in university and institutional labs for fundamental studies in medicinal chemistry, biochemistry, and molecular biology related to peptide function and design.

Basic Information

Product Name	D-Valyl-L-Phenylalanyl-n-(4-Nitrophenyl)-L-Lysinamide Dihydrochloride
CAS No.	74551-31-8
Molecular Formula	C30H44Cl2N6O5
Molecular Weight	639.62 g/mol
Synonyms	D-Val-L-Phe-L-Lys(pNP)-NH2 · 2HCl; H-D-Val-L-Phe-L-Lys(p-nitroanilide)-NH2 dihydrochloride; Nα-(D-Valyl)-Nα-(L-Phenylalanyl)-Nε-(4-Nitrobenzoyl)-L-Lysinamide Dihydrochloride; D-Val-Phe-Lys(pNP)-NH2 · 2HCl; Substrate for Trypsin-like Proteases; p-Nitroanilide (pNA) Peptide Substrate; Chromogenic Peptide Substrate; 74551-31-8
EINECS	Contact for details

Quality Control

Our D-Valyl-L-Phenylalanyl-n-(4-Nitrophenyl)-L-Lysinamide Dihydrochloride is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.