Description

H-Lys(Z)-Amc Hcl is a protected amino acid derivative, specifically Nα-Carbobenzyloxy-L-lysine 7-amido-4-methylcoumarin hydrochloride. This compound serves as a critical fluorogenic substrate and a key synthetic intermediate in advanced biochemical research and pharmaceutical development. It is primarily utilized by researchers and manufacturers in the fields of protease enzymology, peptide synthesis, and high-throughput screening for drug discovery.

Application

• Protease Substrate: Acts as a sensitive fluorogenic substrate for the study and characterization of various proteolytic enzymes, including trypsin, plasmin, and other serine proteases.
• Biochemical Research: Used in enzyme kinetics assays, inhibitor screening, and mechanistic studies to understand protease activity and regulation.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and optimize novel protease inhibitors as potential therapeutic agents.
• Peptide Synthesis Intermediate: Serves as a protected building block (Cbz-Lys) for the solid-phase or solution-phase synthesis of complex peptides and peptide mimetics.
• Diagnostic Reagent Manufacturing: Incorporated into the formulation of specialized diagnostic kits and reagents for clinical and research laboratories.

Basic Information

Product Name	H-Lys(Z)-Amc Hcl
CAS No.	74084-80-3
Molecular Formula	C24H28N3O5Cl
Molecular Weight	473.95 g/mol
Synonyms	Nα-Carbobenzyloxy-L-lysine 7-amido-4-methylcoumarin hydrochloride; Cbz-L-Lysine-AMC hydrochloride; Z-L-Lys-AMC HCl; L-Lysine, N2-[(phenylmethoxy)carbonyl]-, 1-(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl) ester, hydrochloride (1:1); N-Cbz-L-lysine 7-amido-4-methylcoumarin hydrochloride; Fluorogenic substrate for trypsin; AMC-labeled Cbz-L-lysine.
EINECS	Contact for details

Quality Control

Our H-Lys(Z)-Amc Hcl is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing specific results for purity, appearance, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment and handled under conditions that minimize exposure to ambient light and humidity to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.