Description

3'-o-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-Adenosine 5'-Triphosphate is a spin-labeled derivative of adenosine triphosphate (ATP), a critical nucleotide for energy transfer in biological systems. This modified ATP analog is essential for advanced research in structural biology and enzymology, where it serves as a paramagnetic probe for electron paramagnetic resonance (EPR) spectroscopy. It is primarily utilized by research scientists and pharmaceutical developers in biochemistry, molecular biology, and drug discovery to study enzyme kinetics, protein dynamics, and conformational changes in real-time.

Application

• EPR Spectroscopy Studies: Acts as a paramagnetic spin label to investigate the structure, dynamics, and conformational changes of ATP-binding proteins and enzymes.
• Enzyme Kinetics & Mechanism Analysis: Used to probe active sites and monitor real-time enzymatic reactions involving ATP hydrolysis or transfer.
• Membrane Protein Research: Employed in studying ATPases and other membrane-bound transporters where traditional labels are ineffective.
• Drug Discovery & Screening: Serves as a tool in high-throughput assays to identify and characterize inhibitors or modulators of ATP-dependent enzymes.
• Structural Biology: Provides long-range distance constraints in conjunction with NMR or cryo-EM for 3D protein structure determination.
• Biophysical Probes: Used to study protein-protein interactions, allosteric regulation, and folding pathways in complex biological systems.

Basic Information

Product Name	3'-o-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-Adenosine 5'-Triphosphate
CAS No.	74014-72-5
Molecular Formula	C22H33N7O16P3
Molecular Weight	768.46 g/mol
Synonyms	SL-ATP; Spin-Labeled ATP; ATP-SL; 3'-O-(2,2,5,5-Tetramethyl-1-oxyl-3-pyrrolinylcarbonyl)adenosine-5'-triphosphate; 3'-O-(PROXYL-carbonyl)-ATP; ATP-3'-O-(1-oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxylate); Adenosine 5'-triphosphate, 3'-O-[(2,2,5,5-tetramethyl-1-oxido-3-pyrrolin-3-yl)carbonate]; Nitroxide-labeled ATP
EINECS	Contact for details

Quality Control

Our 3'-o-(1-Oxyl-2,2,5,5-Tetramethyl-3-Carbonyl Pyrrolidine)-Adenosine 5'-Triphosphate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and EPR spectroscopy to confirm spin label integrity. Certificates of Analysis (COA) detailing purity, identity, and specific activity are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. The product is hygroscopic (moisture-sensitive) and strictly light-sensitive; all handling should be performed in a controlled, dry environment with minimal exposure to ambient conditions.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (EPR)	Characteristic nitroxide radical signal present
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Specific Activity (Enzymatic Assay)	≥ 90% of theoretical ATP activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.