Description

Boc-Val-Leu-Lys-Amc Acetate is a protected tripeptide fluorogenic substrate, specifically designed for the sensitive detection and study of protease enzyme activity. This compound is critical for biochemical research and pharmaceutical development, where precise measurement of enzymatic kinetics is required. It is primarily utilized by researchers and manufacturers in the fields of proteomics, drug discovery, and diagnostic assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the detection and quantification of specific protease enzymes, including trypsin and related serine proteases.
• High-Throughput Screening (HTS): Used in pharmaceutical research to identify and characterize potential protease inhibitors for therapeutic applications.
• Biochemical Research: A key tool for studying enzyme kinetics, substrate specificity, and catalytic mechanisms in academic and industrial laboratories.
• Diagnostic Development: Employed in the development of sensitive fluorometric diagnostic kits for detecting protease-related biomarkers.
• Cell Biology Studies: Used to monitor protease activity within cellular pathways and processes.

Basic Information

Product Name	Boc-Val-Leu-Lys-Amc Acetate
CAS No.	73554-84-4
Molecular Formula	C33H53N5O8 • C2H4O2
Molecular Weight	Contact for details
Synonyms	Boc-Val-Leu-Lys-AMC Acetate; N-tert-Butoxycarbonyl-L-valyl-L-leucyl-L-lysine 7-amido-4-methylcoumarin Acetate; Boc-VLK-AMC Acetate; Nα-Boc-L-Valyl-L-leucyl-Nε-(7-amino-4-methylcoumarin)-L-lysine Acetate; Fluorogenic Substrate Boc-Val-Leu-Lys-AMC; Protease Substrate V; Substrate for Trypsin-like Proteases
EINECS	Contact for details

Quality Control

Our Boc-Val-Leu-Lys-Amc Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.