Description

Suc-Ala-Phe-Lys-Amc CAS NO 73207-91-7 is a fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic applications for studying enzymes like cathepsins and other serine or cysteine proteases.

Application

• Protease Activity Assays: As a fluorogenic substrate for continuous, real-time monitoring of enzymatic hydrolysis by specific proteases.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibitor screening in academic and industrial R&D settings.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and validate new therapeutic protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in developing diagnostic kits for diseases where protease levels are a biomarker.
• Cell Biology Studies: Applied in live-cell imaging and flow cytometry to detect intracellular protease activity.
• Quality Control: Used in biopharmaceutical manufacturing to monitor the activity and consistency of enzyme-based products.

Basic Information

Product Name	Suc-Ala-Phe-Lys-Amc
CAS No.	73207-91-7
Molecular Formula	C30H39N5O8
Molecular Weight	597.66 g/mol
Synonyms	Succinyl-Ala-Phe-Lys-7-Amino-4-methylcoumarin; Suc-AFK-AMC; Suc-Ala-Phe-Lys-AMC; N-Succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin; Cathepsin B Substrate (Suc-AFK-AMC); Fluorogenic Peptide Substrate Suc-AFK-AMC; N-Succinyl-L-alanyl-L-phenylalanyl-L-lysine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Suc-Ala-Phe-Lys-Amc is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and fluorometric validation for functional activity. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to ensure compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Fluorometric)	≥ 98%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.