Description

Butylagarose is a hydrophobic affinity chromatography medium, specifically engineered by coupling butyl ligands to a cross-linked agarose matrix. This product is essential for the purification and separation of biomolecules, including proteins, enzymes, and antibodies, based on their hydrophobic interactions. It is a critical tool for researchers and process development scientists in the biotechnology, pharmaceutical, and diagnostic industries requiring high-resolution purification protocols.

Application

• Protein Purification: Primary application for the isolation and purification of enzymes, antibodies, and recombinant proteins via hydrophobic interaction chromatography (HIC).
• Biopharmaceutical Downstream Processing: Used in the capture and intermediate purification steps for monoclonal antibodies (mAbs) and other therapeutic proteins.
• Vaccine Development: Employed in the purification of viral antigens and other vaccine components.
• Diagnostic Reagent Production: Facilitates the purification of specific proteins and enzymes used as key components in diagnostic kits and assays.
• Research & Development: A standard medium in academic and industrial labs for studying protein hydrophobicity, stability, and interaction mechanisms.
• Plasma Fractionation: Can be applied in the large-scale purification of albumin, immunoglobulins, and coagulation factors from human plasma.

Basic Information

Product Name	Butylagarose
CAS No.	72980-05-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Butyl-Sepharose; Butyl agarose; Agarose butyl; n-Butyl agarose; Butyl-agarose CL-4B; Hydrophobic agarose butyl; HIC media butyl; Butyl cross-linked agarose; Butyl affinity gel
EINECS	Contact for details

Quality Control

Our Butylagarose is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as ligand density, bead size distribution, flow properties, and binding capacity are strictly controlled. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with each batch to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol or another suitable preservative solution as supplied. Do not freeze. Keep away from strong oxidizing agents.

Specification

Item	Specification
Matrix	Cross-linked 4% Agarose
Ligand	n-Butyl
Bead Size (wet)	45-165 μm
Ligand Density	Contact for details
Maximum Flow Rate	Contact for details
pH Stability (long term)	3 - 13
pH Stability (working)	4 - 12
Storage Solution	20% Ethanol
Appearance	White, spherical beads

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.