Description

Suc-Ala-Pro-Leu-Phe-Pna is a chromogenic peptide substrate designed for the specific detection and quantification of protease activity. This compound is a critical tool in biochemical research and pharmaceutical development, offering high sensitivity and specificity for enzymatic assays. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for studying enzyme kinetics, inhibitor screening, and diagnostic test development.

Application

• Protease Activity Assays: Used as a specific substrate for measuring the activity of chymotrypsin, subtilisin, and related serine proteases.
• Enzyme Kinetic Studies: Essential for determining kinetic parameters (Km, Vmax) and characterizing enzyme mechanisms in academic and industrial research.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening libraries of compounds to identify potential protease inhibitors.
• Diagnostic Development: Serves as a key reagent in the development of colorimetric diagnostic tests for conditions involving abnormal protease levels.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity and purity of enzyme preparations during production processes.
• Biochemical Research: A fundamental tool for investigating protein-protein interactions, signal transduction pathways, and proteolytic cascades.

Basic Information

Product Name	Suc-Ala-Pro-Leu-Phe-Pna
CAS No.	70968-02-4
Molecular Formula	C34H44N6O9
Molecular Weight	680.75 g/mol
Synonyms	Suc-A-P-L-F-pNA; N-Succinyl-Ala-Pro-Leu-Phe-p-nitroanilide; Suc-Ala-Pro-Leu-Phe-pNA; N-Succinyl-Alanyl-Prolyl-Leucyl-Phenylalanyl-p-Nitroanilide; Chymotrypsin Substrate I; Protease Substrate Suc-AAPF-pNA (common related name); Suc-AAPF-pNA; N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (structural analog)
EINECS	Contact for details

Quality Control

Our Suc-Ala-Pro-Leu-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for sensitive research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming identity, purity, and performance in standard assay conditions.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in chymotrypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.