Description

Ac-Ala-Ala-Pro-Phe-Pna is a synthetic, chromogenic peptide substrate designed for enzymatic activity studies. This compound is of significant value in biochemical research and pharmaceutical development for its specificity as a substrate for proteolytic enzymes like chymotrypsin and subtilisin. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics, inhibitor screening, and protease assay development.

Application

• Protease Activity Assays: Used as a specific chromogenic substrate for quantifying the activity of serine proteases, including chymotrypsin, subtilisin, and elastase.
• Enzyme Kinetics Studies: Essential for determining kinetic parameters (Km, Vmax) and studying the mechanism of action of proteolytic enzymes.
• Inhibitor Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Formulation: A key component in the development of clinical diagnostic kits for measuring protease levels in biological samples.
• Biochemical Research: Serves as a tool peptide in academic and industrial laboratories for fundamental research on protein digestion and peptide bond cleavage.
• Quality Control in Biomanufacturing: Used to monitor the presence and activity of specific proteases during downstream bioprocessing.

Basic Information

Product Name	Ac-Ala-Ala-Pro-Phe-Pna
CAS No.	70967-94-1
Molecular Formula	C31H39N7O7
Molecular Weight	621.69 g/mol
Synonyms	N-Acetyl-Ala-Ala-Pro-Phe-p-nitroanilide; Acetyl-Alanyl-Alanyl-Prolyl-Phenylalanyl-p-Nitroanilide; Suc-AAPF-pNA; Ac-AAPF-pNA; Protease Substrate IV; Chymotrypsin Substrate I; N-Acetyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanyl-p-nitroanilide
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Pro-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.