Description

n-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide is a chromogenic peptide substrate specifically designed for the detection and quantification of human leukocyte elastase (HLE) activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by pharmaceutical researchers, diagnostic kit manufacturers, and academic institutions focused on inflammation, pulmonary diseases, and protease inhibitor development.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric and spectrophotometric determination of human leukocyte elastase (HLE) activity in biochemical research.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential elastase inhibitors for therapeutic development.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for conditions associated with elevated elastase levels, such as chronic obstructive pulmonary disease (COPD), cystic fibrosis, and periodontitis.
• Inflammation Research: Enables the study of neutrophil-mediated inflammatory processes and tissue degradation mechanisms in model systems.
• Biochemical Standard: Serves as a reference standard for calibrating assay systems and ensuring reproducibility in enzymatic studies.
• Quality Control in Pharma: Employed in the quality control testing of therapeutic proteins and biologics where residual protease activity must be monitored.

Basic Information

Product Name	n-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide
CAS No.	70967-90-7
Molecular Formula	C24H34N6O9
Molecular Weight	550.57 g/mol
Synonyms	MeOSuc-AAPV-pNA; Methoxysuccinyl-Ala-Ala-Pro-Val-p-Nitroanilide; Suc(OMe)-Ala-Ala-Pro-Val-pNA; Human Leukocyte Elastase Substrate; HLE Substrate; Elastase Substrate IV; N-Methoxysuccinyl-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide; AAPV-pNA
EINECS	Contact for details

Quality Control

Our n-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive enzymatic assays. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.