Description

5,10-Methenyltetrahydrofolic Acid is a key intermediate in the folate metabolic pathway, playing a critical role in one-carbon transfer reactions essential for nucleotide synthesis and amino acid metabolism. This high-purity biochemical is vital for research and development in life sciences and for the synthesis of advanced pharmaceutical compounds. It is primarily utilized by manufacturers and research institutions in the pharmaceutical, biotechnology, and nutraceutical sectors requiring precise and reliable folate derivatives.

Application

• Biochemical Research: Essential as a coenzyme and substrate in enzymatic studies of folate-dependent metabolism, including studies on methylenetetrahydrofolate reductase (MTHFR).
• Pharmaceutical Synthesis: Serves as a critical intermediate in the production of specialized folate-based drugs and active pharmaceutical ingredients (APIs).
• Diagnostic Reagent Manufacturing: Used in the formulation of assay kits and reagents for clinical diagnostics, particularly those measuring folate levels or related metabolic functions.
• Cell Culture & Microbiology: Employed as a growth factor or supplement in defined media for culturing fastidious microorganisms or specialized cell lines.
• Enzyme Cofactor Studies: Applied in research to investigate the mechanisms of enzymes involved in purine, pyrimidine, and amino acid biosynthesis.
• Nutritional Science Research: Used in studies examining folate bioavailability, metabolism, and the development of fortified food products.

Basic Information

Product Name	5,10-Methenyltetrahydrofolic Acid
CAS No.	7444-29-3
Molecular Formula	C20H23N7O6
Molecular Weight	457.44 g/mol
Synonyms	5,10-Methenyl-5,6,7,8-tetrahydrofolic Acid; 5,10-Methenyl-THF; 5,10-CH+-THF; N5,N10-Methenyltetrahydrofolate; Anhydroleucovorin; 5,10-Methenyltetrahydrofolate; 5,10-Methenyl-H4 folate
EINECS	Contact for details

Quality Control

Our 5,10-Methenyltetrahydrofolic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial applications. Certificates of Analysis (COA) documenting full specifications are available upon request, supporting compliance with relevant research and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a cool, dry, and dark place. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.5%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.