Description

L-Leucine β-Naphthylamide is a specialized biochemical reagent primarily used as a chromogenic substrate for the detection and quantification of leucine aminopeptidase (LAP) enzyme activity. This compound matters for its critical role in biochemical research, clinical diagnostics, and enzyme kinetics studies, providing a reliable and sensitive means to measure enzymatic function. It is needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on proteolytic enzyme analysis and biomarker discovery.

Application

• Enzyme Substrate for Leucine Aminopeptidase (LAP): Primary chromogenic substrate used in spectrophotometric assays to measure LAP activity in biological samples.
• Clinical Diagnostics: Key component in diagnostic kits for assessing liver function and certain renal disorders where LAP levels are a relevant biomarker.
• Biochemical Research: Essential tool for studying enzyme kinetics, inhibition, and the characterization of aminopeptidases in vitro.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and evaluate potential enzyme inhibitors as drug candidates.
• Microbiology: Employed in certain bacterial identification systems that rely on specific aminopeptidase profiles.
• Food Science & Quality Control: Applied in enzymatic assays to monitor protein degradation or autolysis processes in food products.

Basic Information

Product Name	L-Leucine β-Naphthylamide
CAS No.	732-85-4
Molecular Formula	C₁₆H₂₀N₂O
Molecular Weight	256.35 g/mol
Synonyms	L-Leucyl-2-naphthylamide; L-Leucyl-β-naphthylamide; Leucyl-2-naphthylamide; N-(2-Naphthyl)-L-leucinamide; LAP Substrate; Leucine 2-naphthylamide; 2-Naphthylamide-L-leucine; L-Leucine-4-(2-naphthyl)azobenzyl ester (related compound)
EINECS	Contact for details

Quality Control

Our L-Leucine β-Naphthylamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by IR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.