Description

4'-C-Azidocytidine 2',3',5'-Tris(2-Methylpropanoate) is a chemically modified nucleoside derivative, specifically a protected form of cytidine with an azido functional group at the 4'-carbon position. This compound is a critical building block (phosphoramidite precursor) in the automated synthesis of oligonucleotides, enabling the site-specific introduction of modifications for advanced research and therapeutic development. It is primarily utilized by pharmaceutical R&D teams, biotech companies, and academic research institutions focused on nucleic acid chemistry, antisense technology, and the development of novel RNA-based therapeutics.

Application

• Oligonucleotide Synthesis: Serves as a key monomer for the solid-phase synthesis of modified DNA/RNA strands, particularly for introducing azide groups for subsequent "click chemistry" conjugations.
• Antisense & siRNA Drug Development: Used to create chemically modified oligonucleotides with enhanced stability, cellular uptake, and target-binding affinity for therapeutic applications.
• Probe and Diagnostic Tool Fabrication: Enables the labeling of nucleic acid probes with fluorescent dyes or other reporter molecules via bioorthogonal reactions for imaging and diagnostic assays.
• Structural Biology Studies: Incorporation into oligonucleotides to study RNA structure, function, and protein-nucleic acid interactions through cross-linking or affinity labeling.
• Materials Science (DNA Nanotechnology): Used as a functional handle to attach oligonucleotides to surfaces, nanoparticles, or polymers for creating advanced biomaterials and biosensors.
• Chemical Biology Research: A versatile tool for exploring metabolic pathways of nucleosides and for the post-synthetic modification of nucleic acids in living systems.

Basic Information

Product Name	4'-C-Azidocytidine 2',3',5'-Tris(2-Methylpropanoate)
CAS No.	690270-29-2
Molecular Formula	C21H28N6O8
Molecular Weight	492.49 g/mol
Synonyms	4'-Azidocytidine tris(pivalate); 4'-N3-Cytidine 2',3',5'-tri-O-pivalate; 1-((2R,3R,4R,5R)-3-Azido-4-(pivaloyloxy)-5-((pivaloyloxy)methyl)tetrahydrofuran-2-yl)-4-(pivaloyloxy)pyrimidin-2(1H)-one; 4'-Azido-2',3',5'-tri-O-pivaloylcytidine; N4-Pivaloyl-4'-azidocytidine 2',3',5'-tripivalate; 4'-C-Azido cytidine protected ester; Azidocytidine pivalate ester
EINECS	Contact for details

Quality Control

Our production of this high-value synthetic intermediate adheres to strict in-process controls to ensure batch-to-batch consistency and purity. Every lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with each shipment to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. The compound is moisture-sensitive and should be handled under an inert atmosphere (argon or nitrogen) when possible to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.