Description

Fmoc-cis-3-Hydroxy-L-Proline CAS NO 648930-78-3 is a protected, non-proteinogenic amino acid derivative essential for advanced peptide synthesis. This compound is a critical building block for introducing constrained, hydroxylated proline motifs into peptide chains, which can significantly influence secondary structure and biological activity. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel therapeutic peptides, peptidomimetics, and chemical biology probes.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the Fmoc/t-Bu strategy, enabling the incorporation of cis-3-hydroxyproline into complex peptide sequences.
• Peptidomimetic Drug Discovery: Used to synthesize conformationally restricted peptide analogs to enhance metabolic stability, bioavailability, and target selectivity for potential therapeutics.
• Glycopeptide and Collagen Mimetic Synthesis: Serves as a precursor for constructing peptides that mimic collagen-like triple helices or incorporate glycosylation sites at the hydroxyproline residue.
• Chemical Biology Probes: Employed in the creation of activity-based probes or labeled peptides to study enzyme function, protein-protein interactions, and cellular pathways.
• Ligand Development for Drug Targets: Integral for designing high-affinity ligands targeting proline-rich motif recognition domains (e.g., SH3, WW domains) or hydroxyproline-specific binding sites.

Basic Information

Product Name	Fmoc-cis-3-Hydroxy-L-Proline
CAS No.	648930-78-3
Molecular Formula	C₂₀H₁₉NO₅
Molecular Weight	353.37 g/mol
Synonyms	Fmoc-cis-3-Hyp-OH; (2S,3R)-1-[(9H-Fluoren-9-ylmethoxy)carbonyl]-3-hydroxypyrrolidine-2-carboxylic Acid; Fmoc-(3R)-hydroxy-L-proline; N-α-Fmoc-cis-3-hydroxy-L-proline; Fmoc-3(R)-hydroxy-L-proline; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-3-hydroxy-L-proline; (2S,3R)-3-Hydroxy-1-{[(9H-fluoren-9-yl)methoxy]carbonyl}pyrrolidine-2-carboxylic acid
EINECS	Contact for details

Quality Control

Our Fmoc-cis-3-Hydroxy-L-Proline is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and chiral integrity, to ensure it meets the high standards required for pharmaceutical research and peptide synthesis. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
Optical Rotation	Contact for details
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.