Description

5-[n-(2-Aminoethyl)-3-(E)-Acrylamido]-5'-o-(Dimethoxytrityl)-2'-Deoxyuridine is a sophisticated, high-purity nucleoside derivative engineered for advanced oligonucleotide synthesis. This compound is a critical building block for the site-specific introduction of functional groups, enabling the creation of modified DNA and RNA sequences with enhanced properties. It is essential for researchers and manufacturers in pharmaceutical development, molecular diagnostics, and biotechnology who require precise and reliable reagents for antisense oligonucleotides, aptamers, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a modified phosphoramidite precursor for the solid-phase synthesis of functionalized DNA and RNA strands.
• Therapeutic Nucleic Acid Development: Key reagent in the production of antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted therapies.
• Probe and Primer Labeling: Enables the covalent attachment of fluorophores, quenchers, or other reporter molecules for diagnostic assays and genomic research.
• Bioconjugation: The reactive acrylamido group facilitates cross-linking to proteins, peptides, or surfaces for creating stable bioconjugates.
• Structural Studies: Used to incorporate specific modifications into nucleic acids for studying structure-function relationships and biomolecular interactions.
• Next-Generation Sequencing (NGS): Can be utilized in the development of specialized sequencing libraries or capture probes.

Basic Information

Item	Details
Product Name	5-[n-(2-Aminoethyl)-3-(E)-Acrylamido]-5'-o-(Dimethoxytrityl)-2'-Deoxyuridine
CAS No.	606126-32-3
Molecular Formula	C₃₇H₄₂N₄O₈
Molecular Weight	670.76 g/mol
Synonyms	5-[N-(2-Aminoethyl)-3-acrylamido]-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine; 5-(3-Acrylamidopropyl)-5'-O-DMT-2'-deoxyuridine (aminoethyl derivative); DMT-dU(AE-ACRYL); Aminoethyl Acrylamido dU DMT; 2'-Deoxy-5-[3-(2-aminoethyl)acrylamido]-5'-O-(4,4'-dimethoxytrityl)uridine; Functionalized dU phosphoramidite precursor; 5-(3-Acrylamido-1-propyl)-5'-O-DMT-2'-deoxyuridine
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive synthetic applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the integrity of the reactive functional groups. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and spectroscopic data is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
DMT Content	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.