Description

S-Hexylglutathione-Sepharose CAS NO 69772-83-4 is a specialized affinity chromatography medium designed for the purification of glutathione S-transferase (GST) and GST-tagged recombinant proteins. This product is critical for ensuring high-purity protein isolation, which is essential for downstream research, diagnostics, and biopharmaceutical development. It is primarily needed by biotechnology companies, academic research institutions, and pharmaceutical manufacturers engaged in proteomics, enzymology, and therapeutic protein production.

Application

• Affinity Purification of GST-Tagged Proteins: The primary application for the rapid, single-step purification of recombinant proteins fused to glutathione S-transferase.
• Enzyme Activity Studies: Used to immobilize GST or related enzymes for kinetic analysis, inhibitor screening, and substrate specificity assays.
• Protein-Protein Interaction Analysis: Serves as a solid support to capture and study protein complexes involving GST-fusion proteins.
• Biopharmaceutical Downstream Processing: Integral in purification trains for the manufacturing of GST-fusion protein therapeutics and vaccines.
• Proteomics Research: Employed in pull-down assays to identify novel binding partners from complex biological mixtures.
• Diagnostic Reagent Production: Used in the purification of enzymes and antigens for immunoassay and biosensor development.

Basic Information

Product Name	S-Hexylglutathione-Sepharose
CAS No.	69772-83-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	S-Hexylglutathione Sepharose 4B; S-Hexylglutathione linked to Sepharose; Glutathione Sepharose analog; Affinity gel for GST; Hexylglutathione-Sepharose; GST Purification Resin; S-(Hexyl)glutathione-Sepharose 4 Fast Flow; Glutathione derivative affinity medium
EINECS	Contact for details

Quality Control

Our S-Hexylglutathione-Sepharose is manufactured under strict quality management systems. Each batch undergoes rigorous testing for ligand density, binding capacity, flow characteristics, and absence of microbial contamination to ensure consistent performance for critical separations. Certificates of Analysis (COA) detailing these specifications are available upon request, supporting compliance in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied storage solution (typically 20% ethanol). Do not freeze. Keep away from strong acids, strong bases, and organic solvents. The product is light-sensitive and should be stored away from direct light exposure.

Specification

Item	Specification
Appearance	White to off-white slurry
Matrix	Sepharose 4B
Ligand	S-Hexylglutathione
Binding Capacity	>10 mg recombinant GST per ml settled gel
Ligand Density	Contact for details
Particle Size	45-165 μm
Storage Solution	20% Ethanol
pH Stability	3-12 (long term), 2-13 (short term)
Microbial Bioburden	Absent

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.