Description

n-Acetylphenylalanylarginine Ethyl Ester is a high-purity, synthetic peptide derivative designed for advanced research and development applications. This compound serves as a critical building block and biochemical tool, offering precise molecular structure for studying enzyme-substrate interactions and metabolic pathways. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and fine chemical manufacturers focused on protease research, peptide synthesis, and the development of novel therapeutic agents.

Application

• Biochemical Research: Used as a specific substrate or inhibitor in enzymatic assays, particularly for studying serine proteases like trypsin and related enzymes.
• Pharmaceutical Development: Acts as a key intermediate in the synthesis of more complex peptide-based drug candidates and pharmacologically active compounds.
• Protease Mechanism Studies: Employed to investigate enzyme kinetics, specificity, and catalytic mechanisms due to its well-defined acetylated N-terminus and arginine side chain.
• Diagnostic Reagent Formulation: Incorporated into in-vitro diagnostic kits and reagents for clinical testing and biochemical analysis.
• Peptide Library Construction: Serves as a building block in combinatorial chemistry and solid-phase peptide synthesis for creating diverse molecular libraries.
• Academic & Industrial R&D: A standard reagent in laboratories for teaching advanced biochemistry concepts and for internal R&D projects in biotechnology.

Basic Information

Product Name	n-Acetylphenylalanylarginine Ethyl Ester
CAS No.	69536-83-0
Molecular Formula	C20H30N6O5
Molecular Weight	434.49 g/mol
Synonyms	Ac-Phe-Arg-OEt; N-Acetyl-L-phenylalanyl-L-arginine ethyl ester; Nα-Acetyl-L-phenylalanyl-L-arginine ethyl ester; APAE; Acetylphenylalanylarginine ethyl ester; L-Arginine, N2-[N-(acetyl-L-phenylalanyl)]-, ethyl ester; Ethyl N-acetyl-L-phenylalanyl-L-argininate; Trypsin substrate APAE
EINECS	Contact for details

Quality Control

Every batch of n-Acetylphenylalanylarginine Ethyl Ester is manufactured under strict quality management protocols. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing purity, chiral purity, and impurity profiles is provided with each shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent exposure to atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.