Description

L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters for researchers and quality control labs requiring precise, real-time detection of protease activity, particularly trypsin and related enzymes. It is essential for professionals in pharmaceutical R&D, diagnostic kit manufacturing, and biochemical research who need reliable, high-purity substrates for kinetic studies and inhibitor screening.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and quantification of trypsin, thrombin, and other arginine-specific proteases.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring enzymatic activity in biological samples.
• Biochemical Research: Enables real-time kinetic analysis of enzyme mechanisms, substrate specificity, and inhibition constants (Ki) in academic and industrial labs.
• Quality Control in Bioprocessing: Monitors protease activity during upstream and downstream biomanufacturing processes to ensure product consistency.
• Cell Biology Studies: Applied in assays to detect and localize protease activity within live cells or tissue sections.

Basic Information

Product Name	L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride
CAS No.	69304-16-1
Molecular Formula	C20H28N6O4 • 2HCl
Molecular Weight	505.40 g/mol (free base: 416.48)
Synonyms	L-Arginine-7-amido-4-methylcoumarin dihydrochloride; Arg-AMC • 2HCl; L-Arg-AMC dihydrochloride; 7-Amino-4-methylcoumarin conjugate of L-Arginine dihydrochloride; AMC-L-Arginine dihydrochloride; Boc-L-Arg-AMC • 2HCl (common precursor); Fluorogenic trypsin substrate; Protease substrate AMC-Arg.
EINECS	Contact for details

Quality Control

Our L-Arginine 7-Amido-4-Methylcoumarin Dihydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectrophotometric verification of functional performance. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and assay results is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Assay (Fluorometric)	≥ 95.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.