Description

ε-Tnp-L-Lysine Hydrochloride is a chemically modified derivative of the essential amino acid L-lysine, specifically functionalized with a trinitrophenyl (Tnp) group at the ε-amino position. This modification is critical for its primary role as a highly specific active site inhibitor and affinity label for enzymes and proteins that utilize lysine residues. It is an indispensable research-grade biochemical for scientists in pharmaceutical discovery and proteomics, enabling precise mechanistic studies and structural analysis. The compound is essential for academic, biotech, and pharmaceutical R&D laboratories focused on enzyme kinetics, protein chemistry, and the development of targeted therapeutics.

Application

• Enzyme Inhibition Studies: Used as a covalent, active-site-directed inhibitor for enzymes like transglutaminases, lysyl oxidases, and other lysine-dependent enzymes to elucidate catalytic mechanisms.
• Affinity Chromatography & Protein Purification: Serves as a key ligand for preparing affinity columns to isolate and purify specific proteins or antibody fractions that bind to modified lysine epitopes.
• Bioconjugation & Probe Development: Acts as a starting material or intermediate for synthesizing fluorescent probes, cross-linkers, or other bioconjugates for cellular imaging and protein interaction studies.
• Pharmaceutical Research: Employed in high-throughput screening (HTS) assays and target validation during early-stage drug discovery for conditions involving aberrant protein cross-linking or modification.
• Biochemical Assay Standards: Functions as a reference standard or calibrant in quantitative analytical methods such as HPLC or mass spectrometry for related biochemical pathways.
• Academic Research & Teaching: A valuable tool in university laboratories for teaching advanced concepts in enzymology and chemical biology.

Basic Information

Product Name	ε-Tnp-L-Lysine Hydrochloride
CAS No.	69002-98-8
Molecular Formula	C15H22N4O6 • HCl
Molecular Weight	398.82 g/mol
Synonyms	Nε-Tnp-L-lysine hydrochloride; N6-(2,4,6-Trinitrophenyl)-L-lysine hydrochloride; L-Lysine, N6-(2,4,6-trinitrophenyl)-, hydrochloride; TNP-lysine HCl; ε-TNP-L-lysine HCl; 2,4,6-Trinitrophenyl-L-lysine hydrochloride; Affinity label for lysine sites
EINECS	Contact for details

Quality Control

Our ε-Tnp-L-Lysine Hydrochloride is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and IR for structural confirmation, and specific optical rotation verification. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Specific Optical Rotation	+15° to +25° (c=1 in H2O)
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.