Description

Z-Gly-Pro-Arg-Pna is a chromogenic peptide substrate specifically designed for the determination of thrombin activity. This compound is a critical reagent in biochemical research and diagnostic applications, offering high sensitivity and specificity for enzymatic assays. It is primarily utilized by research institutions, pharmaceutical developers, and clinical diagnostic laboratories involved in hemostasis and thrombosis studies, protease research, and drug discovery.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric or spectrophotometric measurement of thrombin concentration and activity in research and diagnostic kits.
• Hemostasis Research: Used in studies of blood coagulation pathways, inhibitor screening, and the investigation of thrombotic disorders.
• Pharmaceutical Development: Essential for the high-throughput screening (HTS) of potential anticoagulant or procoagulant drug candidates targeting thrombin.
• Quality Control in Biologics: Employed to test for residual thrombin activity in the production of fibrin sealants and other biopharmaceuticals.
• Enzyme Kinetics: Serves as a specific substrate for detailed kinetic studies of thrombin's enzymatic mechanism and inhibition constants (Ki).
• Diagnostic Reagent Manufacturing: A key component in the formulation of standardized clinical diagnostic tests for coagulation factor deficiencies.

Basic Information

Product Name	Z-Gly-Pro-Arg-Pna
CAS No.	66648-35-9
Molecular Formula	C27H34N8O7
Molecular Weight	582.61 g/mol
Synonyms	Benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide; Z-Gly-Pro-Arg-pNA; Chromozym TH; Thrombin Substrate I; Tosyl-Gly-Pro-Arg-p-nitroanilide (common alternative); S-2238 (similar commercial substrate); H-D-Phe-Pip-Arg-pNA (related compound); Boc-Val-Pro-Arg-pNA (related compound)
EINECS	Contact for details

Quality Control

Our Z-Gly-Pro-Arg-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing in thrombin assays. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Assay (Functional)	≥ 95% of theoretical activity
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.