Description

Biotin Hydrazide CAS NO 66640-86-6 is a high-purity chemical derivative of biotin (Vitamin B7) featuring a reactive hydrazide group. This functional group makes it a critical intermediate for the synthesis of biotinylated probes, affinity tags, and other conjugates used in advanced biochemical research and diagnostics. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require reliable, high-quality building blocks for developing targeted assays, diagnostic kits, and novel therapeutic agents.

Application

• Bioconjugation & Probe Synthesis: Key intermediate for covalently attaching biotin to antibodies, proteins, nucleic acids, and other molecules to create detection probes.
• Affinity Chromatography Ligands: Used to synthesize biotinylated ligands for immobilization on streptavidin/avidin resins for purification processes.
• Diagnostic Kit Manufacturing: A vital raw material in the production of ELISA, lateral flow assays, and other immunoassay components.
• Chemical Biology Research: Employed in the study of biomolecular interactions, cell surface labeling, and pull-down assays.
• Pharmaceutical Intermediate: Serves as a precursor in the synthesis of more complex biotin-derived active pharmaceutical ingredients (APIs).
• Material Science: Can be used to functionalize surfaces and nanomaterials with biotin for biosensor development.

Basic Information

Item	Details
Product Name	Biotin Hydrazide
CAS No.	66640-86-6
Molecular Formula	C10H18N4O2S
Molecular Weight	258.34 g/mol
Synonyms	Biotin hydrazide; Biotinoyl hydrazide; D-Biotin hydrazide; Vitamin B7 hydrazide; Vitamin H hydrazide; (+)-Biotin hydrazide; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic hydrazide
EINECS	Contact for details

Quality Control

Our Biotin Hydrazide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, IR and NMR for structural confirmation, and tests for residual solvents and moisture. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure traceability and compliance with your research or production specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent degradation. For long-term storage, consider storing under an inert atmosphere to minimize oxidation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.