Description

Hemoglobin I Philadelphia CAS NO 66369-37-7 is a naturally occurring variant of human hemoglobin, characterized by a specific amino acid substitution in the β-globin chain. This protein is of significant interest in biochemical and clinical research for studying the structure-function relationships of hemoglobin and the pathophysiology of hemoglobinopathies. It serves as a critical reference material for researchers and diagnostic manufacturers in the fields of hematology, molecular diagnostics, and pharmaceutical development.

Application

• Biochemical Research Standard: Used as a high-purity reference standard for studying oxygen-binding kinetics, protein stability, and allosteric regulation in hemoglobin variants.
• Clinical Diagnostics Development: Essential for the calibration and validation of diagnostic assays and instrumentation designed to detect and quantify abnormal hemoglobins.
• Pharmaceutical R&D: Employed in drug discovery programs targeting sickle cell disease and other hemoglobin disorders to evaluate therapeutic efficacy.
• Hematology Studies: Serves as a model compound for investigating the correlation between specific genetic mutations and their phenotypic expression in red blood cells.
• Educational & Reference Material: Supplied to academic and institutional laboratories for advanced teaching and proteomics research.

Basic Information

Product Name	Hemoglobin I Philadelphia
CAS No.	66369-37-7
Molecular Formula	C2952H4664N812O832S40Fe4
Molecular Weight	~64,500 Da (Tetramer)
Synonyms	Hb I Philadelphia; Hemoglobin I (Philadelphia); Hb Philly; Hemoglobin variant Philly; α2β235 Tyr→Phe; Hemoglobin alpha 2 beta 2 35(C1) Tyr→Phe; Unstable hemoglobin Philadelphia; HBB: c.106T>A (p.Tyr36Phe)
EINECS	Contact for details

Quality Control

Our Hemoglobin I Philadelphia is produced and handled under strict quality protocols to ensure batch-to-batch consistency and integrity. Each lot is characterized using advanced analytical techniques including HPLC, electrophoresis, and spectrophotometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, spectral properties, and physical form is provided with every shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (HPLC/Electrophoresis)	Conforms to standard
Purity (HPLC)	≥ 95%
Assay (Spectrophotometric)	≥ 90% of theoretical
Iron Content	0.30 - 0.34% (w/w)
Solubility	Soluble in buffer or water
Residual Moisture (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.