Description

n-Biotinyl-1,6-Hexanediamine is a high-purity, synthetic biotin derivative where the vitamin is conjugated to a hexanediamine spacer arm via its carboxyl group. This molecular design is critical for introducing biotin's strong binding affinity to avidin and streptavidin into various target molecules, enabling precise detection, isolation, and labeling. It is an essential intermediate and reagent for researchers and manufacturers in the life sciences, particularly for developing advanced diagnostic assays, targeted drug delivery systems, and bioconjugation platforms.

Application

• Bioconjugation & Probe Synthesis: Used as a linker to biotinylate antibodies, peptides, nucleic acids, and other biomolecules for use in ELISA, Western blotting, and flow cytometry.
• Diagnostic Kit Manufacturing: A key raw material in the production of lateral flow assays, immunoassay reagents, and other in-vitro diagnostic (IVD) components.
• Drug Delivery & Targeting: Serves as a building block for creating biotinylated drug conjugates and ligands for avidin-biotin technology-based targeted therapies.
• Affinity Chromatography: Employed in the synthesis of biotinylated capture ligands immobilized on avidin/streptavidin resins for protein purification.
• Cell Surface Labeling: Facilitates the tagging of cell surface receptors and other membrane proteins for imaging and sorting applications.
• Material Science & Nanotechnology: Functionalizes nanoparticles, polymers, and surfaces with biotin for creating smart biomaterials and biosensors.

Basic Information

Product Name	n-Biotinyl-1,6-Hexanediamine
CAS No.	65953-56-2
Molecular Formula	C16H30N4O2S
Molecular Weight	342.50 g/mol
Synonyms	Biotin-XX hydrazide (common misnomer for amine); Biotinamidocaproyl hydrazide (common misnomer); Biotin-LC-hydrazide; 6-[(5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyl)amino]hexylamine; Biotinamidohexanoic acid hydrazide; N-Biotinyl-1,6-diaminohexane; Biotinylated hexanediamine; (+)-Biotinyl-6-aminocaproic acid hydrazide
EINECS	Contact for details

Quality Control

Our n-Biotinyl-1,6-Hexanediamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants or under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.