Description

Kemptide CAS NO 65189-71-1 is a synthetic peptide substrate specifically designed for the assay of cyclic AMP-dependent protein kinase (PKA). This compound is essential for biochemical research and pharmaceutical development, providing a reliable and sensitive tool for measuring PKA activity. It is primarily used by researchers and quality control laboratories in the biotechnology, pharmaceutical, and academic sectors for enzyme kinetics studies, drug discovery screening, and diagnostic assay development.

Application

• Protein Kinase A (PKA) Activity Assays: Primary substrate for the quantitative measurement of PKA enzymatic activity in vitro.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel PKA inhibitors or activators for therapeutic development.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, phosphorylation mechanisms, and signal transduction pathways involving cAMP.
• Diagnostic Kit Development: A key component in the formulation of commercial assay kits for research and clinical diagnostic applications.
• Quality Control in Biomanufacturing: Employed to monitor and validate the activity of PKA enzymes used in various bioprocesses.
• Academic & Teaching Laboratories: Used in university settings for practical experiments in biochemistry and molecular biology courses.

Basic Information

Item	Detail
Product Name	Kemptide
CAS No.	65189-71-1
Molecular Formula	C38H64N12O12
Molecular Weight	857.0 g/mol
Synonyms	Leu-Arg-Arg-Ala-Ser-Leu-Gly; L-Leucyl-L-arginyl-L-arginyl-L-alanyl-L-seryl-L-leucyl-glycine; PKA Substrate Peptide; cAMP-dependent Protein Kinase Substrate; Protein Kinase A (PKA) Substrate; LRRASLG; Synthetic PKA Substrate
EINECS	Contact for details

Quality Control

Our Kemptide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. We ensure compliance with rigorous internal specifications suitable for research and in vitro diagnostic use. A Certificate of Analysis (COA) detailing purity, assay results, and physical characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Peptide Content)	≥ 80%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.