Description

Boc-Ile-Glu-Gly-Arg-Amc Acetate Salt is a protected peptide sequence featuring a C-terminal fluorogenic amide (Aminomethylcoumarin, AMC) group. This compound is a critical substrate for the study and characterization of proteolytic enzymes, particularly serine and cysteine proteases. It is essential for researchers and manufacturers in the fields of pharmaceutical development, biochemical research, and diagnostic assay production, where precise enzyme activity measurement is required.

Application

• Protease Activity Assays: Primary substrate for high-throughput screening (HTS) and kinetic studies of trypsin-like serine proteases and other enzymes that cleave after arginine residues.
• Biochemical Research: Used in mechanistic studies to determine enzyme specificity, inhibition constants (Ki), and catalytic efficiency.
• Pharmaceutical R&D: Employed in drug discovery programs to identify and characterize novel protease inhibitors for therapeutic targets.
• Diagnostic Development: Serves as a key component in the development of fluorogenic diagnostic kits for clinical and research laboratories.
• Enzyme Characterization: Essential tool for the purification and functional analysis of recombinant or isolated proteases.
• Quality Control: Used in biomanufacturing to monitor the activity and consistency of enzyme batches.

Basic Information

Product Name	Boc-Ile-Glu-Gly-Arg-Amc Acetate Salt
CAS No.	65147-06-0
Molecular Formula	C₃₄H₅₃N₉O₁₁ • C₂H₄O₂
Molecular Weight	Contact for details
Synonyms	Boc-Ile-Glu-Gly-Arg-AMC Acetate; t-Butyloxycarbonyl-Isoleucine-Glutamic Acid-Glycine-Arginine-7-Amino-4-Methylcoumarin Acetate; Boc-IEGR-AMC Acetate; Fluorogenic Protease Substrate Boc-IEGR-AMC; Ac-Ile-Glu-Gly-Arg-AMC (common alias); Protease Substrate V; Thrombin Substrate I (related application); 7-Amino-4-methylcoumarin derivative
EINECS	Contact for details

Quality Control

Every batch of Boc-Ile-Glu-Gly-Arg-Amc Acetate Salt is manufactured under strict quality management protocols. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.