Description

H-Ala-Pro-Pna Hcl CAS NO 65022-17-5 is a high-purity, synthetic peptide derivative used as a critical biochemical reagent and research compound. Its primary value lies in its role as a specific chromogenic substrate for the detection and study of dipeptidyl peptidase IV (DPP-IV) and related serine proteases. This product is essential for researchers and manufacturers in the fields of pharmaceutical development, biochemical research, and diagnostic assay production, where precise enzyme activity measurement is required.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of DPP-IV (CD26) activity in research and diagnostic applications.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential DPP-IV inhibitors for drug discovery programs targeting type 2 diabetes and other conditions.
• Biochemical Research: A key tool for studying the kinetics, specificity, and function of proline-specific peptidases in metabolic and immunological pathways.
• Diagnostic Kit Manufacturing: Incorporated as a core component in commercial and clinical diagnostic kits for measuring DPP-IV levels in serum or plasma samples.
• Cell Biology Studies: Employed to investigate the role of DPP-IV in T-cell activation, cancer biology, and signal transduction processes.
• Quality Control: Serves as a reference standard for the quality assurance of other enzyme substrates and related biochemical reagents.

Basic Information

Product Name	H-Ala-Pro-Pna Hcl
CAS No.	65022-17-5
Molecular Formula	C14H19N5O3 • HCl
Molecular Weight	341.79 g/mol (Free base: 305.33 g/mol)
Synonyms	Ala-Pro-p-Nitroanilide hydrochloride; H-Ala-Pro-pNA•HCl; L-Alanyl-L-prolyl-4-nitroanilide hydrochloride; DPP-IV Substrate; Dipeptidyl Peptidase IV Substrate; Ala-Pro-4-NA•HCl; Gly-Pro-pNA (common related substrate); Alanyl-prolyl-p-nitroanilide hydrochloride
EINECS	Contact for details

Quality Control

Every batch of H-Ala-Pro-Pna Hcl is manufactured and tested under strict quality management systems to ensure consistency and reliability for critical research applications. Our standard grade meets high-purity specifications suitable for enzymatic assays and biochemical research. Certificates of Analysis (COA) are provided with each shipment, detailing purity, identity, and other critical parameters. We can support development projects requiring compliance with GMP or ISO guidelines upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation from atmospheric moisture. For long-term storage over 12 months, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in DMSO or buffer
Biological Activity	Suitable for DPP-IV enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.