Description

Prolyl-Phenylalanyl-Arginine-4-Nitroanilide is a chromogenic peptide substrate specifically designed for the detection and kinetic study of proteolytic enzyme activity. This compound is of critical importance in biochemical research and diagnostic assay development, where precise measurement of enzyme kinetics is required. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology and protease inhibitor screening.

Application

• Protease Activity Assays: As a selective substrate for measuring the activity of specific serine or cysteine proteases in research settings.
• Diagnostic Kit Development: Used in the formulation of in-vitro diagnostic (IVD) kits for clinical analysis of blood coagulation factors and other medically relevant proteases.
• Enzyme Kinetic Studies: Enables the determination of kinetic parameters (Km, Vmax, kcat) for target enzymes in biochemical characterization.
• High-Throughput Screening (HTS): Utilized in pharmaceutical discovery for screening libraries of compounds to identify novel protease inhibitors.
• Quality Control in Biologics: Monitoring the presence and activity of proteases during the production and purification of biopharmaceuticals.
• Biochemical Research: Fundamental research tool for studying protease specificity, mechanism of action, and regulatory pathways.

Basic Information

Item	Detail
Product Name	Prolyl-Phenylalanyl-Arginine-4-Nitroanilide
CAS No.	64816-19-9
Molecular Formula	C30H41N9O7
Molecular Weight	639.71 g/mol
Synonyms	Pro-Phe-Arg-pNA; H-Pro-Phe-Arg-p-nitroanilide; PFR-pNA; Chromogenic Substrate PFR-pNA; Pro-Phe-Arg-4-nitroanilide; L-Prolyl-L-phenylalanyl-L-arginine 4-nitroanilide; S-2765 (related chromogenic substrate series); Thrombin Chromogenic Substrate (related application)
EINECS	Contact for details

Quality Control

Our Prolyl-Phenylalanyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and spectrophotometric verification of functional performance. Certificates of Analysis (COA) detailing identity, purity, and assay results are provided and available upon request to ensure compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Absorbance Spectrum	Conforms (λmax ~315 nm in suitable solvent)
Functional Activity	≥ 95% of reference standard in specified assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.