Description

Hexa-His CAS NO 64134-30-1 is a high-purity synthetic peptide sequence, specifically a hexamer of the amino acid histidine. This compound is a critical reagent in biotechnology and life sciences research, primarily valued for its strong and specific affinity for immobilized metal ions. It is essential for researchers and manufacturers in the biopharmaceutical sector, particularly for the purification of recombinant proteins via immobilized metal affinity chromatography (IMAC).

Application

• Primary tag for the purification of recombinant fusion proteins using Immobilized Metal Affinity Chromatography (IMAC).
• Critical component in the development and production of biopharmaceuticals, including monoclonal antibodies and therapeutic proteins.
• Used in proteomics and genomics research for studying protein-protein interactions and protein function.
• Essential reagent in diagnostic kit development, particularly for assays requiring high-specificity capture molecules.
• Applied in the creation of biosensors and bio-conjugates where a stable, high-affinity binding site is required.
• Utilized in academic and industrial research for structural biology studies, including X-ray crystallography and NMR spectroscopy.

Basic Information

Product Name	Hexa-His
CAS No.	64134-30-1
Molecular Formula	C36H50N18O6
Molecular Weight	806.90 g/mol
Synonyms	Hexahistidine; Polyhistidine-tag; His6-tag; 6xHis tag; HHHHHH; Histidine Hexamer; (His)6; Polyhistidine Peptide; Histidine Oligomer; Metal Chelating Peptide
EINECS	Contact for details

Quality Control

Our Hexa-His is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and MS for identity confirmation, to ensure it meets the high standards required for research and bioprocessing. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (Content)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.