Description

L-Glutamic Acid γ-(3-Carboxy-4-Nitroanilide) Ammonium Salt is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its high specificity and sensitivity in detecting and quantifying protease activity, particularly for enzymes like glutamyl endopeptidases. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in biochemical research, diagnostic development, and drug discovery.

Application

• Biochemical Research: As a specific substrate for studying the kinetics and inhibition of glutamyl endopeptidases and related proteolytic enzymes.
• Diagnostic Assays: A key component in the development of colorimetric or fluorogenic diagnostic kits for detecting specific protease biomarkers.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitor drug candidates.
• Enzyme Characterization: For determining the specific activity, purity, and functional integrity of enzyme preparations in quality control laboratories.
• Microbiology: Potential use in assays to study bacterial proteases involved in pathogenesis or industrial processes.

Basic Information

Product Name	L-Glutamic Acid γ-(3-Carboxy-4-Nitroanilide) Ammonium Salt
CAS No.	63699-78-5
Molecular Formula	C14H18N4O7 • xNH3
Molecular Weight	Contact for details
Synonyms	L-Glutamic Acid γ-(3-Carboxy-4-Nitroanilide) Ammonium Salt; Glutaryl-3-Carboxy-4-Nitroanilide Ammonium Salt; GpNA (Glutaryl-p-nitroanilide) derivative; N-Glutaryl-3-Carboxy-4-Nitroaniline Ammonium Salt; Substrate for Glutamyl Endopeptidase; 3-Carboxy-4-Nitroanilide of L-Glutamic Acid Ammonium Salt; Protease Substrate 63699-78-5
EINECS	Contact for details

Quality Control

Our L-Glutamic Acid γ-(3-Carboxy-4-Nitroanilide) Ammonium Salt is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.