Description

Carboxymethyl Sephadex CAS NO 62886-59-3 is a functionalized derivative of the cross-linked dextran gel, Sephadex, featuring carboxymethyl groups that introduce cation-exchange properties. This modification is critical for enabling the separation and purification of biomolecules, particularly proteins and peptides, based on differences in their charge. It is an essential tool for researchers and process engineers in the biotechnology and pharmaceutical industries who require high-resolution ion-exchange chromatography for downstream processing, analytical applications, and laboratory-scale purification.

Application

• Ion-Exchange Chromatography (IEC): Primary matrix for the separation and purification of proteins, enzymes, peptides, and other charged biomolecules in both analytical and preparative scales.
• Biopharmaceutical Downstream Processing: Used in purification trains for monoclonal antibodies, recombinant proteins, vaccines, and other therapeutic biologics.
• Laboratory Research & Development: A key tool for biochemical research, including enzyme isolation, plasma fractionation, and the study of protein-protein interactions.
• Desalting and Buffer Exchange: Can be utilized for the removal of salts or exchange of buffers from protein solutions during various purification steps.
• Enzyme Immobilization: Serves as a support matrix for the covalent immobilization of enzymes via its carboxyl groups, facilitating biocatalysis and enzyme reuse.
• Diagnostic Reagent Manufacturing: Employed in the purification of specific antigens or antibodies used in diagnostic kits and assays.

Basic Information

Product Name	Carboxymethyl Sephadex
CAS No.	62886-59-3
Molecular Formula	(C6H10O5)n with -CH2COOH substituents
Molecular Weight	Polymeric substance
Synonyms	CM-Sephadex; Carboxymethyl Sephadex Ion Exchanger; Sephadex CM; Carboxymethylated Sephadex; Dextran, 2-(carboxymethyl) ether, cross-linked; Cross-linked carboxymethyl dextran; Cation exchanger Sephadex; CM-Sephadex C-25; CM-Sephadex C-50
EINECS	Contact for details

Quality Control

Our Carboxymethyl Sephadex is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including ion-exchange capacity, particle size distribution, swelling factor, and absence of microbial contamination. A comprehensive Certificate of Analysis (COA) detailing these specifications is provided with every shipment. We support applications requiring adherence to cGMP and ISO 9001 standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Ensure the container is sealed tightly after each use to prevent moisture uptake, which can affect swelling properties and performance. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Ion-Exchange Capacity	4.0 - 5.0 meq/g (typical)
Particle Size (wet)	40 - 120 μm (Fractionated grades available)
Swelling Factor (H2O)	6 - 10 mL/g
pH Stability Range	2 - 10 (operational)
Functional Group	Carboxymethyl (-CH2COOH)
Matrix	Cross-linked dextran
Counter Ion	Na+ (Sodium form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.