Description

H-Lys(Biotinyl)-NH2 is a specialized biotinylated lysine derivative, a crucial building block in peptide synthesis and biochemical research. This compound is essential for introducing a biotin tag onto peptides and proteins, enabling their detection, purification, and immobilization via the high-affinity streptavidin-biotin interaction. It is primarily used by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for applications such as affinity chromatography, immunoassays, and targeted drug delivery systems.

Application

• Peptide Synthesis: A key intermediate for the solid-phase or solution-phase synthesis of biotin-labeled peptides.
• Affinity Probes: Used to create biotinylated probes for studying protein-protein interactions, receptor binding, and signal transduction pathways.
• Diagnostic Assays: Critical component in the development of ELISA, lateral flow assays, and other diagnostic kits that utilize biotin-streptavidin chemistry for signal amplification.
• Drug Delivery Systems: Facilitates the conjugation of therapeutic agents to targeting ligands via biotin-avidin bridges for site-specific delivery.
• Bioconjugation & Immobilization: Enables the stable attachment of proteins, antibodies, or enzymes to avidin/streptavidin-coated surfaces, beads, or chips.
• Pull-Down Assays: Used to biotinylate bait proteins for isolation and identification of interacting partners from complex biological mixtures.

Basic Information

Product Name	H-Lys(Biotinyl)-NH2
CAS No.	61125-53-9
Molecular Formula	C16H28N4O3S
Molecular Weight	356.48 g/mol
Synonyms	Biotin-Lysine; Nε-Biotinyl-L-lysine; N6-Biotinyl-L-lysine; L-Lysine, N6-biotinyl-; Biotinyl-lysine; Lys(Biotin)-NH2; H-Lys(Biotin)-NH2; Biotinylated Lysine
EINECS	Contact for details

Quality Control

Our H-Lys(Biotinyl)-NH2 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and chiral analysis to ensure enantiomeric purity. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee consistent quality and traceability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be kept under inert atmosphere or desiccated upon opening for long-term storage to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Optical Rotation	-10.0° to -14.0° (c=1 in 1N HCl)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.