Description

Benzyloxycarbonylalanyl-Alanyl-Phenylalanine-4-Nitroanilide is a synthetic, chromogenic peptide substrate designed for high-precision enzymatic assays. This compound is critical for researchers and quality control laboratories requiring accurate measurement of protease and peptidase activity. It is primarily utilized in the pharmaceutical, biotechnology, and biochemical research sectors for enzyme kinetics studies, inhibitor screening, and diagnostic kit development.

Application

• Protease Activity Assays: A standard substrate for quantifying the activity of various serine and cysteine proteases in research and development.
• Enzyme Kinetics & Inhibitor Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical and research laboratories.
• Biochemical Research: Employed in mechanistic studies to understand substrate specificity and catalytic mechanisms of peptidases.
• Quality Control (QC) Testing: Used in pharmaceutical QC to monitor the presence and activity of specific enzymes during bioprocessing.

Basic Information

Product Name	Benzyloxycarbonylalanyl-Alanyl-Phenylalanine-4-Nitroanilide
CAS No.	61043-35-4
Molecular Formula	C30H32N6O7
Molecular Weight	588.61 g/mol
Synonyms	Z-Ala-Ala-Phe-pNA; Z-AAP-pNA; Cbz-Ala-Ala-Phe-pNA; Benzyloxycarbonyl-Ala-Ala-Phe-4-nitroanilide; N-[(Benzyloxy)carbonyl]-L-alanyl-L-alanyl-N4-(4-nitrophenyl)-L-phenylalaninamide; Substrate for Chymotrypsin; Chromogenic Peptide Substrate; AAPF-pNA
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylalanyl-Alanyl-Phenylalanine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.