Description

H-Asp-Pro-Pna is a synthetic peptide derivative containing the aspartic acid-proline dipeptide sequence linked to a p-nitroaniline (Pna) moiety. This compound is a specialized biochemical tool of significant value in proteomics and enzymology research, primarily serving as a chromogenic substrate for specific proteases. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require high-purity, reliable substrates for enzyme activity assays, kinetic studies, and diagnostic kit development.

Application

• Protease/Enzyme Substrate: Used as a specific chromogenic substrate for the detection and quantification of protease enzyme activity, where cleavage releases yellow-colored p-nitroaniline.
• Biochemical Research: Employed in kinetic studies, enzyme inhibition assays, and mechanistic investigations of aspartic acid and proline-specific proteases.
• Diagnostic Kit Development: A key component in the formulation of diagnostic assays and kits for clinical and research laboratories to measure specific protease levels.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitor drug candidates.
• Quality Control in Bioprocessing: Applied in monitoring protease activity during upstream and downstream biomanufacturing processes to ensure product consistency.

Basic Information

Product Name	H-Asp-Pro-Pna
CAS No.	60189-48-2
Molecular Formula	C15H19N5O6
Molecular Weight	365.35 g/mol
Synonyms	Aspartic Acid-Proline p-Nitroanilide; Asp-Pro-pNA; H-Asp-Pro-p-Nitroanilide; L-Aspartyl-L-prolyl 4-nitroanilide; Substrate for Cathepsin D/E; Protease Substrate 60189-48-2; N-(4-Nitrophenyl)-L-α-aspartyl-L-prolinamide
EINECS	Contact for details

Quality Control

Our H-Asp-Pro-Pna is manufactured under controlled conditions to ensure high batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for biochemical and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.