Description

S-(p-Nitrobenzyl)Glutathione is a synthetic glutathione derivative where the thiol group is specifically alkylated with a p-nitrobenzyl moiety. This compound is a critical biochemical tool and a key intermediate in the study of glutathione S-transferase (GST) activity and detoxification pathways. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, biochemical research, and diagnostic assay production. Its well-defined structure makes it essential for enzyme kinetics studies, inhibitor screening, and as a standard in analytical methods.

Application

• Biochemical Research: A standard substrate for assaying the activity of Glutathione S-Transferase (GST) enzymes in vitro.
• Pharmaceutical Development: Used in high-throughput screening (HTS) to identify and characterize potential GST inhibitors for therapeutic applications.
• Diagnostic Kits: Serves as a key reagent in commercial and research ELISA kits and other assays designed to measure GST activity in biological samples.
• Metabolic Pathway Studies: Employed to investigate Phase II detoxification mechanisms and cellular defense against electrophilic toxins and oxidative stress.
• Reference Standard: Used as a certified reference material (CRM) or analytical standard in HPLC, LC-MS, and other chromatographic methods for quality control and method validation.
• Enzyme Purification: Can be used in affinity chromatography for the purification of specific GST isoenzymes.

Basic Information

Product Name	S-(p-Nitrobenzyl)Glutathione
CAS No.	6803-19-6
Molecular Formula	C17H23N4O8S
Molecular Weight	443.45 g/mol
Synonyms	S-(4-Nitrobenzyl)glutathione; γ-Glu-Cys-Gly-S-CH2-C6H4-NO2; Glutathione S-(p-nitrobenzyl) ester; L-γ-Glutamyl-L-cysteinylglycine S-(4-nitrobenzyl) thioether; GST Substrate (p-nitrobenzyl); 4-Nitrobenzyl glutathione; p-Nitrobenzyl glutathione
EINECS	Contact for details

Quality Control

Our S-(p-Nitrobenzyl)Glutathione is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our quality system is designed to meet the exacting standards required for reliable and reproducible scientific and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.