Description

Abz-Phe-Arg-Lys(Dnp)-Pro-Oh is a specialized, high-purity peptide substrate designed for advanced biochemical research and diagnostic applications. This compound is critical for studying and quantifying the activity of specific proteolytic enzymes, particularly in complex biological systems. It serves as an essential tool for researchers and developers in the fields of pharmaceutical discovery, clinical diagnostics, and proteomics, where precise enzyme kinetics and inhibition studies are paramount.

Application

• Protease Activity Assays: Primary substrate for fluorometric and colorimetric assays to measure the activity of specific serine proteases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Essential reagent for kinetic studies, enzyme mechanism elucidation, and substrate specificity profiling in academic and industrial laboratories.
• Diagnostic Kit Development: Key component in the formulation of diagnostic kits for detecting and monitoring diseases associated with dysregulated protease activity.
• Quality Control in Biologics: Employed in the quality control of biopharmaceuticals to assess the presence and activity of residual process-related proteases.

Basic Information

Product Name	Abz-Phe-Arg-Lys(Dnp)-Pro-Oh
CAS No.	500799-61-1
Molecular Formula	C46H58N12O11
Molecular Weight	931.04 g/mol
Synonyms	Abz-FRK(Dnp)-P-OH; o-Aminobenzoyl-Phe-Arg-Lys(2,4-dinitrophenyl)-Pro-OH; Fluorogenic Peptide Substrate for Proteases; FRET-based Protease Substrate; 2-Aminobenzoyl-L-phenylalanyl-L-arginyl-Nε-(2,4-dinitrophenyl)-L-lysyl-L-proline; Protease Substrate V; DABCYL-FRET Substrate Analog
EINECS	Contact for details

Quality Control

Our Abz-Phe-Arg-Lys(Dnp)-Pro-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specified parameters to ensure the material meets the exacting standards required for sensitive research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.