Description

L-Proline-15N is a stable isotope-labeled analog of the amino acid L-proline, where the nitrogen atom is enriched with the 15N isotope. This compound is essential for advanced research and analytical applications requiring precise molecular tracking and quantification. It is primarily utilized by scientists and manufacturers in the pharmaceutical, biotechnology, and life science sectors for applications such as metabolic pathway studies, protein NMR spectroscopy, and mass spectrometry-based proteomics.

Application

• Nuclear Magnetic Resonance (NMR) Spectroscopy: Used as an isotopic tracer for structural elucidation and dynamics studies of proteins, peptides, and other biomolecules.
• Mass Spectrometry & Quantitative Proteomics: Serves as an internal standard for accurate quantification of proline-containing peptides and proteins in metabolic flux analysis and SILAC (Stable Isotope Labeling by Amino acids in Cell culture) experiments.
• Metabolic Research: Employed to trace and study proline metabolism, collagen biosynthesis, and related biochemical pathways in cellular and animal models.
• Pharmaceutical Development: Used in the research and development of new drugs, particularly in pharmacokinetic and metabolic stability studies of proline-based compounds.
• Biochemical Reagent: Acts as a high-purity component in the formulation of cell culture media, diagnostic kits, and enzymatic assays requiring defined isotopic content.
• Academic & Industrial Research: A critical tool for basic research in biochemistry, molecular biology, and medicinal chemistry within university, government, and corporate R&D laboratories.

Basic Information

Product Name	L-Proline-15N
CAS No.	59681-31-1
Molecular Formula	C₅H₉¹⁵NO₂
Molecular Weight	116.12 g/mol (¹⁵N)
Synonyms	(2S)-Pyrrolidine-2-carboxylic acid-15N; L-(-)-Proline-15N; Proline-15N; (S)-Proline-15N; (-)-Proline-15N; 15N-L-Proline; 15N Labeled L-Proline; Isotope L-Proline (15N)
EINECS	Contact for details

Quality Control

Our L-Proline-15N is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 15N), chemical purity, and specific analytical results. We support compliance with relevant research and development standards, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 15N)	≥ 98%
Optical Rotation	-84° to -86° (c=1 in H₂O)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.