Description

n-Benzoyl-Pro-Phe-Arg p-Nitroanilide Hydrochloride is a high-purity chromogenic substrate specifically designed for the sensitive and quantitative determination of trypsin, thrombin, and related serine proteases. This compound is critical for biochemical research and diagnostic applications where precise enzyme activity measurement is required. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology, hemostasis, and protease inhibitor screening.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric or fluorometric determination of trypsin, thrombin, plasmin, and other trypsin-like serine proteases.
• Diagnostic Development: Key component in the formulation of clinical diagnostic kits for monitoring coagulation disorders, pancreatitis, and other conditions involving protease activity.
• Pharmaceutical Research: Used in high-throughput screening (HTS) platforms to identify and characterize novel serine protease inhibitors for drug discovery.
• Biochemical Research: Essential tool for studying enzyme kinetics, specificity, and inhibition mechanisms in academic and industrial laboratories.
• Quality Control: Employed in the quality assurance of enzyme preparations and biopharmaceutical products where protease activity must be quantified.

Basic Information

Product Name	n-Benzoyl-Pro-Phe-Arg p-Nitroanilide Hydrochloride
CAS No.	59188-28-2
Molecular Formula	C32H36ClN9O6
Molecular Weight	678.14 g/mol
Synonyms	Bz-Pro-Phe-Arg pNA · HCl; Chromogenic Substrate S-2302 (Kabi); H-D-Pro-Phe-Arg-pNA · HCl; Benzoyl-L-prolyl-L-phenylalanyl-L-arginine 4-nitroanilide hydrochloride; Bz-Pro-Phe-Arg-pNA hydrochloride; Thrombin Substrate I; Trypsin Substrate; Tos-Gly-Pro-Arg-pNA (common related substrate); BPR-pNA · HCl
EINECS	Contact for details

Quality Control

Our n-Benzoyl-Pro-Phe-Arg p-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be kept in a dry environment. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in water or DMSO
Functional Activity	Conforms to reference in standard trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.