Description

Digitonin is a steroidal saponin glycoside derived from the seeds of the foxglove plant, *Digitalis purpurea*. This high-purity biochemical reagent is valued for its unique ability to selectively permeabilize cell membranes, specifically cholesterol-containing membranes, without solubilizing them. It is an essential tool for researchers and manufacturers in life sciences, particularly for cell biology studies, protein extraction, and diagnostic applications. Our supply of Digitonin CAS NO 59033-71-5 ensures consistent quality and reliability for critical industrial and research processes.

Application

• Cell Membrane Permeabilization: Selective permeabilization of plasma membranes for intracellular antibody staining in flow cytometry (FACS) and immunofluorescence microscopy.
• Mitochondrial Isolation: Key reagent in protocols for the gentle isolation of intact mitochondria from mammalian cells and tissues.
• Protein Extraction & Solubilization: Used to solubilize membrane-bound proteins, including GPCRs (G-protein-coupled receptors), for structural and functional studies.
• Cholesterol Assay & Research: Serves as a specific cholesterol-binding agent in biochemical assays to quantify or sequester cholesterol.
• Diagnostic Reagent Manufacturing: A component in the formulation of specific diagnostic kits and biochemical reagents.
• Detergent in Crystallography: Employed as a mild detergent for the crystallization of membrane proteins.
• Signal Transduction Studies: Facilitates the study of intracellular signaling pathways by allowing controlled access to the cell interior.

Basic Information

Product Name	Digitonin
CAS No.	59033-71-5
Molecular Formula	C56H92O29
Molecular Weight	1229.31 g/mol
Synonyms	Digitonin; Digitalin; (3β,5β,25R)-3-[(2,6-Dideoxy-4-O-β-D-glucopyranosyl-β-D-ribo-hexopyranosyl)oxy]-14-hydroxycard-20(22)-enolide; Digitalis saponin; Digitonide; Digitonine; Strophanthidin 3-tri-digitoxoside; EINECS 261-585-1
EINECS	261-585-1

Quality Control

Our Digitonin is produced and tested under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and tests for residual solvents and heavy metals. We provide full traceability and Certificates of Analysis (COA) are available for all shipments, ensuring compliance with research and industrial grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Residue on Ignition (Sulfated Ash)	≤ 0.1 %
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in DMSO or Ethanol)	Clear, colorless to faint yellow solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.